In-spray chamber hydride generation by multi-mode sample introduction system (MSIS) as an interface in the hyphenated system of high performance liquid chromatography and inductivity coupled plasma optical emission spectrometry (HPLC/HG-ICP-OES) in arsenic species determination.

The paper presents a new usage of a commercial in-spray chamber hydride generator, Multi-mode Sample Introduction System (MSIS) as a connector of high pressure liquid chromatography and inductivity coupled plasma optical emission spectrometry (HPLC/HG-ICP-OES). This hyphenated technique was applied in a determination of inorganic and organic arsenic species. The optimization of a hydride generation process in MSIS and a chromatographic separation (using anion-exchange HPLC column with phosphate buffer as a mobile phase) were carried out compositely. The method allowed to determine three arsenic forms, As(III), As(V) and dimethylarsinate (DMA) within 400s of a single analysis. The obtained limits of detection (LODs) were 2.08   μg L-1 for As(III); 6.97   μg L-1 for As(V); 6.15   μg L-1 for DMA and precision 2.7%, 4.8%, and 5.7%, respectively, for 100   μg L-1. The described method was used for environmental and food samples analyses.