Ling-Sai Chang1,2, Mindy Ming-Huey Guo1,2,3, Mao-Hung Lo1, Ho-Chang Kuo4,5,6. 1. Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan. 2. Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. 3. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan. 4. Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan. erickuo48@yahoo.com.tw. 5. Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. erickuo48@yahoo.com.tw. 6. Department of Respiratory Therapy, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, 83301, Taiwan. erickuo48@yahoo.com.tw.
Abstract
BACKGROUND: Kawasaki disease (KD) is associated with expression and methylation of Fc gamma receptor genes. We characterized immunoglobulin A (IgA), IgE, IgG, and IgM receptor expression levels in KD. METHODS: Fc receptor expression levels were characterized using GeneChip Human Transcriptome Array 2.0 (HTA 2.0) with 18 KD patients, 18 non-febrile controls, and 18 febrile controls. Another 48 control individuals and 46 patients with KD were measured using pyrosequencing for the methylation levels. RESULTS: The mRNA expression levels of FCER1A and FCER2 were significantly lower in KD patients than in non-febrile controls and then rose following treatments with intravenous immunoglobulin (IVIG). Expression levels of FCER1G increased compared to the non-febrile subjects and then subsided after IVIG. FCER1A methylation was significantly lower among KD patients and even lower in KD patients with IVIG resistance. HTA analysis revealed higher mRNA levels of FCAR, FCGR1C, and FCGR2A in KD patients. FCMR mRNA expression levels were significantly lower in KD patients. FCMR expression levels rose after IVIG treatment. After IVIG, FCGR1A, B, and C decreased even lower than the febrile controls. CONCLUSION: This is the first study indicating that IgA, IgE, IgG, and IgM receptors are associated with KD. We highlighted potential biomarkers related to Fc receptors and their regulation.
BACKGROUND:Kawasaki disease (KD) is associated with expression and methylation of Fc gamma receptor genes. We characterized immunoglobulin A (IgA), IgE, IgG, and IgM receptor expression levels in KD. METHODS: Fc receptor expression levels were characterized using GeneChip Human Transcriptome Array 2.0 (HTA 2.0) with 18 KDpatients, 18 non-febrile controls, and 18 febrile controls. Another 48 control individuals and 46 patients with KD were measured using pyrosequencing for the methylation levels. RESULTS: The mRNA expression levels of FCER1A and FCER2 were significantly lower in KDpatients than in non-febrile controls and then rose following treatments with intravenous immunoglobulin (IVIG). Expression levels of FCER1G increased compared to the non-febrile subjects and then subsided after IVIG. FCER1A methylation was significantly lower among KDpatients and even lower in KDpatients with IVIG resistance. HTA analysis revealed higher mRNA levels of FCAR, FCGR1C, and FCGR2A in KDpatients. FCMR mRNA expression levels were significantly lower in KDpatients. FCMR expression levels rose after IVIG treatment. After IVIG, FCGR1A, B, and C decreased even lower than the febrile controls. CONCLUSION: This is the first study indicating that IgA, IgE, IgG, and IgM receptors are associated with KD. We highlighted potential biomarkers related to Fc receptors and their regulation.
Authors: A Robin Temming; Matthias Tammes Buirs; Arthur E H Bentlage; Louise W Treffers; Hannah Feringa; Steven W de Taeye; Taco W Kuijpers; Sietse Q Nagelkerke; Giso Brasser; Juk Yee Mok; Wim J E van Esch; Timo K van den Berg; Theo Rispens; C Ellen van der Schoot; Gestur Vidarsson Journal: Front Immunol Date: 2021-03-15 Impact factor: 7.561
Authors: Ling-Sai Chang; Jia-Huei Yan; Jin-Yu Li; Deniz Des Yeter; Ying-Hsien Huang; Mindy Ming-Huey Guo; Mao-Hung Lo; Ho-Chang Kuo Journal: Int J Environ Res Public Health Date: 2020-05-25 Impact factor: 3.390