A laccase based biosensor on AuNPs-MoS2 modified glassy carbon electrode for catechol detection.

As a kind of two-dimensional layered nanomaterial, MoS2 nanosheets have been widely used in the field of biosensors. In this work, a novel laccase based biosensor for catechol detection was fabricated based on a nanocomposite of MoS2 nanosheets and gold nanoparticles. The experimental results demonstrated that MoS2 had large specific surface area and good biocompatibility, which provided abundant position for enzyme immobilization. Gold nanoparticles enhanced conductivity of MoS2 and improved detection sensitivity. Because of the synergistic effect of MoS2 nanosheets and gold nanoparticles, the laccase based bioelectrode exhibited a linear response to catechol from 2 to 2000 μM with a detection limit of 2 μM (S/N = 3), as well as good selectivity, stability, repeatability, and reproducibility.