Polydopamine-PEG-Folic Acid Conjugate Film Engineered TiO2 Nanotube Arrays for Photoelectrochemical Sensing of Folate Binding Protein.

Serum-soluble folate binding protein (FBP) is an important tumor marker and the development of simple biosensing method is highly needed. In this work, a photoelectrochemical (PEC) biosensor for the detection of FBP was proposed based on the construction of an antifouling interface and the unique ligand-protein recognition. The PEC sensing platform was prepared by the biomimetic polydopamine (PDA) coating on TiO2 nanotubes arrays (NTAs). Significant PEC enhancement effect was obtained due to the macro porous structures. Excellent antifouling performance was achieved by the conjugation of amino-group terminated 8-arm poly(ethylene glycol) (PEG). The incorporation of folic acid (FA) retains the antifouling property and shows recognition abilities towards FBP. The fabricated PEC biosensor show good analytical performances. The combination of ligand-protein recognition and PEC antifouling interface provides a good consideration for the development of FBP biosensors.