Linzhen Xie1, Jinwu Wang1, Yingying Zhang2, Hua Chen1, Dingsheng Lin1, Jian Ding1, Jiangwei Xuan1, Qi Chen1, Leyi Cai1. 1. Department of Orthopaedics Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University NO. 109, Xueyuan West Road, Luheng District, Wenzhou 325000, Zhejiang Province, P. R. China. 2. Department of Radiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University NO. 109, Xueyuan West Road, Luheng District, Wenzhou 325000, Zhejiang Province, P. R. China.
Abstract
PURPOSE: To determine if the viability of random pattern dorsal skin flaps in rats could be improved with local injection of exosomes derived from bone marrow mesenchymal stem cells (BMSCs). METHODS: 30 adult male SD rats (weight 200-250 g) were randomly divided into experimental and control groups. Exosomes were isolated from the 4th generation of BMSCs. Experimental group were treated with local injection of exosomes suspension while control group were treated with saline solution in the same way. McFarlane-type flaps (9.0 × 3.0 cm) were then operated in the dorsum of all. On the seventh postoperative day, the percentage of viability area was measured and the blood flow was detected by laser doppler. Then rats were sacrificed and flaps were cut off for further check. RESULTS: Compared with controls, the exosomes increased the area of survival (P<0.05). Hematoxylin and eosin (HE) staining of zone II showed higher microvascular density (P<0.05) and better angiogenesis (P<0.05) in the exosomes group. Similarly, the blood flow of exosomes was better than the control group according to laser doppler imaging (P<0.05). And the result of immunohistochemistry and western blot showed that the exosomes group had significantly higher VEGF and CD34 expression compared to the controls (P<0.05). CONCLUSIONS: Local injection of BMSCs exosomes was effective to attenuate necrosis of the McFarlane-type flaps in rats. AJTR
PURPOSE: To determine if the viability of random pattern dorsal skin flaps in rats could be improved with local injection of exosomes derived from bone marrow mesenchymal stem cells (BMSCs). METHODS: 30 adult male SD rats (weight 200-250 g) were randomly divided into experimental and control groups. Exosomes were isolated from the 4th generation of BMSCs. Experimental group were treated with local injection of exosomes suspension while control group were treated with saline solution in the same way. McFarlane-type flaps (9.0 × 3.0 cm) were then operated in the dorsum of all. On the seventh postoperative day, the percentage of viability area was measured and the blood flow was detected by laser doppler. Then rats were sacrificed and flaps were cut off for further check. RESULTS: Compared with controls, the exosomes increased the area of survival (P<0.05). Hematoxylin and eosin (HE) staining of zone II showed higher microvascular density (P<0.05) and better angiogenesis (P<0.05) in the exosomes group. Similarly, the blood flow of exosomes was better than the control group according to laser doppler imaging (P<0.05). And the result of immunohistochemistry and western blot showed that the exosomes group had significantly higher VEGF and CD34 expression compared to the controls (P<0.05). CONCLUSIONS: Local injection of BMSCs exosomes was effective to attenuate necrosis of the McFarlane-type flaps in rats. AJTR
Authors: Ruenn Chai Lai; Fatih Arslan; May May Lee; Newman Siu Kwan Sze; Andre Choo; Tian Sheng Chen; Manuel Salto-Tellez; Leo Timmers; Chuen Neng Lee; Reida Menshawe El Oakley; Gerard Pasterkamp; Dominique P V de Kleijn; Sai Kiang Lim Journal: Stem Cell Res Date: 2010-01-04 Impact factor: 2.020
Authors: Jason M Aliotta; Mandy Pereira; Sicheng Wen; Mark S Dooner; Michael Del Tatto; Elaine Papa; Laura R Goldberg; Grayson L Baird; Corey E Ventetuolo; Peter J Quesenberry; James R Klinger Journal: Cardiovasc Res Date: 2016-03-14 Impact factor: 10.787