Amelioration of chromium and heat stresses in Sorghum bicolor by Cr6+ reducing-thermotolerant plant growth promoting bacteria.

Climatic factors particularly increased atmospheric temperature (IAT) greatly alters plant microbe and heavy metal interactions and subsequently reduces plant growth and phytoremediation efficiency. The aim of the study was to assess the effects of inoculation of chromium reducing-thermotolerant plant growth promoting bacteria (CRT-PGPB) on plant growth, physiological responses and chromium (Cr) uptake by Sorghum bicolor under IAT condition. Three potential CRT-PGPB strains were isolated from Cr contaminated sites and identified as Bacillus cereus TCR17, Providencia rettgeri TCR21 and Myroides odoratimimus TCR22 through molecular characterization. These strains displayed the potential to reduce Cr6+ to Cr3+, produce siderophores, indole-3-acetic acid and solubilize phosphate. Inoculation of S. bicolor with CRT-PGPB increased plant growth, antioxidant status (superoxide dismutase, catalase and ascorbate peroxidase) and decreased proline and malondialdehyde contents in plants under Cr, IAT and Cr + IAT stress indicate that PGPB helped plants to reduce stress induced oxidative damage. Irrespective of IAT stress, inoculation of CRT-PGPB decreased the accumulation of Cr in plants compared with un-inoculated control suggest that CRT-PGPB might have the potential to improve phytostabilization process in Cr contaminated soils. Furthermore, gene expression studies confirmed that inoculation of TCR21 down-regulated the expression of proline synthesis gene (p5cs1) and up-regulated the expression of antioxidant related genes (sod, apx1 and cat) and stress tolerance genes (sHsp). Our results showed that CRT-PGPB exhibiting potential to tolerate Cr, temperature, produce plant beneficial metabolites and reduce Cr6+ to Cr3+, can be exploited as potential inoculants for improving plant growth and phytoremediation process in Cr contaminated soil under IAT condition.