Kriti Soni1, Ali Mujtaba2, Md Habban Akhter3, Ameeduzzafar Zafar4, Kanchan Kohli1. 1. Department of Pharmaceutics, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi-110062, India. 2. Departments of Pharmaceutics, Faculty of Pharmacy, Northern Border University, Rafha, Kingdom of Saudi Arabia. 3. Faculty of Pharmacy, DIT University, Dehradun-248009, India. 4. Department of Pharmaceutics, College of pharmacy, Jouf University, Aljouf, KSA.
Abstract
Aim: The optimization and evaluation of ethosomal nanogel (NGs) for topical delivery in skin cancer. Methods: The formulations were optimized by employing 3-factor, 3-level Box Behnken design for responses of vesicle size, and fluxes. They characterized in vitro and evaluated for drug release, permeation and retention, skin penetration of ethosome, electron microscopy, texture analysis, and in vitro cytotoxicity. Results: The optimized formulation exhibited z-average 125.67 ± 10.43 nm, apparent zeta potential -17.1 ± 2.61 mV, average flux of drug loaded ethosome were 54.72 ± 5.45 and 59.83 ± 6.09 µg/cm2/h. Further, Rhodamine B loaded ethosome penetrated deeper up to 183.82 µm. The NGs texture analysis showed index of viscosity 225.45 g.s, firmness 209.34 g, cohesiveness -189.48 g, and consistency 59.45 g.s. The optimized ethosome NGs exhibited significant anti-cancer effect in B16-F10 murine tumor cell line (p < 0.05). Conclusion: Ethosomal NGs could be promising for skin cancer treatment.
Aim: The optimization and evaluation of ethosomal nanogel (NGs) for topical delivery in skin cancer. Methods: The formulations were optimized by employing 3-factor, 3-level Box Behnken design for responses of vesicle size, and fluxes. They characterized in vitro and evaluated for drug release, permeation and retention, skin penetration of ethosome, electron microscopy, texture analysis, and in vitro cytotoxicity. Results: The optimized formulation exhibited z-average 125.67 ± 10.43 nm, apparent zeta potential -17.1 ± 2.61 mV, average flux of drug loaded ethosome were 54.72 ± 5.45 and 59.83 ± 6.09 µg/cm2/h. Further, Rhodamine B loaded ethosome penetrated deeper up to 183.82 µm. The NGs texture analysis showed index of viscosity 225.45 g.s, firmness 209.34 g, cohesiveness -189.48 g, and consistency 59.45 g.s. The optimized ethosome NGs exhibited significant anti-cancer effect in B16-F10 murinetumor cell line (p < 0.05). Conclusion:Ethosomal NGs could be promising for skin cancer treatment.