Literature DB >> 3180462

Immunoaffinity purification of human erythrocyte prolidase.

F Endo1, A Tanoue, T Ogata, K Motohara, I Matsuda.   

Abstract

A procedure including immunoaffinity gel chromatography of an immobilized monoclonal antibody was used to isolate human erythrocyte prolidase (EC 3.4.13.9). The monoclonal antibody was developed against liver prolidase and the antibody recognized the erythrocyte enzyme. The purification procedure included three steps of DEAE cellulose (batcher), immunoaffinity gel chromatography and gel filtration column chromatography. The overall recovery was approximately 20% and the specific activity of the purified preparation was approximately 260 U/mg of protein, a value exceeding that obtained using conventional procedures.

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Year:  1988        PMID： 3180462     DOI： 10.1016/0009-8981(88)90201-x

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  4 in total

1.  Biochemical basis of prolidase deficiency. Polypeptide and RNA phenotypes and the relation to clinical phenotypes.

Authors:  F Endo; A Tanoue; A Kitano; J Arata; D M Danks; C M Lapière; Y Sei; S K Wadman; I Matsuda
Journal:  J Clin Invest       Date:  1990-01       Impact factor: 14.808

2.  Deduced amino acid sequence of human prolidase and molecular analyses of prolidase deficiency.

Authors:  F Endo; A Tanoue; A Hata; A Kitano; I Matsuda
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

3.  Molecular defect in siblings with prolidase deficiency and absence or presence of clinical symptoms. A 0.8-kb deletion with breakpoints at the short, direct repeat in the PEPD gene and synthesis of abnormal messenger RNA and inactive polypeptide.

Authors:  A Tanoue; F Endo; I Akaboshi; T Oono; J Arata; I Matsuda
Journal:  J Clin Invest       Date:  1991-04       Impact factor: 14.808

Review 4.  Clinical Genetics of Prolidase Deficiency: An Updated Review.

Authors:  Marta Spodenkiewicz; Michel Spodenkiewicz; Maureen Cleary; Marie Massier; Giorgos Fitsialos; Vincent Cottin; Guillaume Jouret; Céline Poirsier; Martine Doco-Fenzy; Anne-Sophie Lèbre
Journal:  Biology (Basel)       Date:  2020-05-21
  4 in total

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