Elif Fırat1, Zafer Aybek2, Şakir Akgün3, Kürşat Küçüker2, Hakan Akça4, Hülya Aybek5. 1. Department of Medical Biochemistry, Pamukkale University School of Medicine, Denizli, Turkey. elifbasak43@hotmail.com. 2. Department of Urology, Pamukkale University School of Medicine, Denizli, Turkey. 3. Department of Medical Biology, Kafkas University School of Medicine, Kars, Turkey. 4. Department of Medical Genetics, Pamukkale University School of Medicine, Denizli, Turkey. 5. Department of Medical Biochemistry, Pamukkale University School of Medicine, Denizli, Turkey.
Abstract
PURPOSE: Adrenergic and cholinergic pathways play an important role in contraction-relaxation harmony in human bladder. Functional changes in any proteins in these pathways may result in overactive bladder. We aimed to investigate whether single gene polymorphisms affecting adrenergic and cholinergic pathways are associated with OAB syndrome. METHODS: 60 patients with idiopathic OAB and 60 healthy controls were included in the study. A validated OAB-V8 questionnaire was given to all patients. Polymorphisms of ADRB3, ROCK2, and GEF gene were detected by PCR from whole blood samples. Genotypic structures of patients and controls were compared. The relationship between genotypic structures and OAB symptom scores were investigated. RESULTS: We found no significant difference in the genotype and allele frequencies between the patients and controls for all three SNP. While there was no relationship between ADRB3 and GEF gene polymorphisms and OAB scores in OAB patients, the OAB score in heterozygous polymorphic individuals was significantly higher than in homozygous polymorphic individuals in the ROCK2 gene (p = 0.039). CONCLUSION: The polymorphisms of the ADRB3, ROCK2, and GEF genes were present in both OAB group and healthy controls, but were not associated with OAB syndrome.
PURPOSE: Adrenergic and cholinergic pathways play an important role in contraction-relaxation harmony in human bladder. Functional changes in any proteins in these pathways may result in overactive bladder. We aimed to investigate whether single gene polymorphisms affecting adrenergic and cholinergic pathways are associated with OAB syndrome. METHODS: 60 patients with idiopathic OAB and 60 healthy controls were included in the study. A validated OAB-V8 questionnaire was given to all patients. Polymorphisms of ADRB3, ROCK2, and GEF gene were detected by PCR from whole blood samples. Genotypic structures of patients and controls were compared. The relationship between genotypic structures and OAB symptom scores were investigated. RESULTS: We found no significant difference in the genotype and allele frequencies between the patients and controls for all three SNP. While there was no relationship between ADRB3 and GEF gene polymorphisms and OAB scores in OAB patients, the OAB score in heterozygous polymorphic individuals was significantly higher than in homozygous polymorphic individuals in the ROCK2 gene (p = 0.039). CONCLUSION: The polymorphisms of the ADRB3, ROCK2, and GEF genes were present in both OAB group and healthy controls, but were not associated with OAB syndrome.
Authors: L J Emorine; S Marullo; M M Briend-Sutren; G Patey; K Tate; C Delavier-Klutchko; A D Strosberg Journal: Science Date: 1989-09-08 Impact factor: 47.728
Authors: K Clément; C Vaisse; B S Manning; A Basdevant; B Guy-Grand; J Ruiz; K D Silver; A R Shuldiner; P Froguel; A D Strosberg Journal: N Engl J Med Date: 1995-08-10 Impact factor: 91.245