Wei Zhou1, Jun Nie1, Dafa Zhang1. 1. Department of Cardiothoracic Surgery, First Affiliated Hospital of Wannan Medical College, Wuhu 241001, China.
Abstract
OBJECTIVE: To investigate the differential expression of miR-126-5p in patients with lung adenocarcinoma and explore the possible mechanism. METHODS: We searched STARBASE database to analyze the differential expression of miR-126-5p between lung adenocarcinoma tissues and normal lung tissues. The prognosis of patients with lung adenocarcinoma was analyzed on Kaplan-Meier Plotter online website, and the survival curves of the patients with different expression levels of miR-126-5p were generated. The target gene of mir-126-5p was predicted by STARBASE database, and the expression level of the target gene and its influence on the patients' prognosis were analyzed using online website tool. We also examined the expression levels of miR-126-5p in peripheral serum of 30 healthy control subjects and 30 patients with lung adenocarcinoma using qPCR. RESULTS: Analysis of the data from STARBASE database showed a high expression of miR-126-5p in normal lung tissues but a low expression in lung adenocarcinoma tissues. Kaplan-Meier Plotter online analysis based on big data analysis showed that patients with a high expression of miR-126-5p had a better survival prognosis than those with a low expression (HR=0.68, P=0.015). MiR-126-5p was predicted to bind to the 3'UTR region of BRCC3 mRNA, and their expression levels were negatively correlated (r=0.197, P < 0.05). Compared with normal tissues, lung adenocarcinoma tissues expressed high levels of BRCC3, which was associated with a poor prognosis of the patients (HR=1.39, P < 0.05). The serum level of miR-126-5p was significantly higher in healthy control subjects than in patients with lung adenocarcinoma (1.23 ± 0.21 vs 0.63 ± 0.12, P < 0.05). CONCLUSIONS: The expression level of miR-126-5p is lowered in lung adenocarcinoma tissue, and patients with lung adenocarcinoma have lower serum level of miR-126-5p than healthy subjects. A high expression of miR-126-5p is associated with a more favorable prognosis of the patients than a low expression. miR-126-5p may play a role against cancer by regulating BRCC3.
OBJECTIVE: To investigate the differential expression of miR-126-5p in patients with lung adenocarcinoma and explore the possible mechanism. METHODS: We searched STARBASE database to analyze the differential expression of miR-126-5p between lung adenocarcinoma tissues and normal lung tissues. The prognosis of patients with lung adenocarcinoma was analyzed on Kaplan-Meier Plotter online website, and the survival curves of the patients with different expression levels of miR-126-5p were generated. The target gene of mir-126-5p was predicted by STARBASE database, and the expression level of the target gene and its influence on the patients' prognosis were analyzed using online website tool. We also examined the expression levels of miR-126-5p in peripheral serum of 30 healthy control subjects and 30 patients with lung adenocarcinoma using qPCR. RESULTS: Analysis of the data from STARBASE database showed a high expression of miR-126-5p in normal lung tissues but a low expression in lung adenocarcinoma tissues. Kaplan-Meier Plotter online analysis based on big data analysis showed that patients with a high expression of miR-126-5p had a better survival prognosis than those with a low expression (HR=0.68, P=0.015). MiR-126-5p was predicted to bind to the 3'UTR region of BRCC3 mRNA, and their expression levels were negatively correlated (r=0.197, P < 0.05). Compared with normal tissues, lung adenocarcinoma tissues expressed high levels of BRCC3, which was associated with a poor prognosis of the patients (HR=1.39, P < 0.05). The serum level of miR-126-5p was significantly higher in healthy control subjects than in patients with lung adenocarcinoma (1.23 ± 0.21 vs 0.63 ± 0.12, P < 0.05). CONCLUSIONS: The expression level of miR-126-5p is lowered in lung adenocarcinoma tissue, and patients with lung adenocarcinoma have lower serum level of miR-126-5p than healthy subjects. A high expression of miR-126-5p is associated with a more favorable prognosis of the patients than a low expression. miR-126-5p may play a role against cancer by regulating BRCC3.
Authors: Timothy R Rebbeck; Nandita Mitra; Fei Wan; Olga M Sinilnikova; Sue Healey; Lesley McGuffog; Sylvie Mazoyer; Georgia Chenevix-Trench; Douglas F Easton; Antonis C Antoniou; Katherine L Nathanson; Yael Laitman; Anya Kushnir; Shani Paluch-Shimon; Raanan Berger; Jamal Zidan; Eitan Friedman; Hans Ehrencrona; Marie Stenmark-Askmalm; Zakaria Einbeigi; Niklas Loman; Katja Harbst; Johanna Rantala; Beatrice Melin; Dezheng Huo; Olufunmilayo I Olopade; Joyce Seldon; Patricia A Ganz; Robert L Nussbaum; Salina B Chan; Kunle Odunsi; Simon A Gayther; Susan M Domchek; Banu K Arun; Karen H Lu; Gillian Mitchell; Beth Y Karlan; Christine Walsh; Jenny Lester; Andrew K Godwin; Harsh Pathak; Eric Ross; Mary B Daly; Alice S Whittemore; Esther M John; Alexander Miron; Mary Beth Terry; Wendy K Chung; David E Goldgar; Saundra S Buys; Ramunas Janavicius; Laima Tihomirova; Nadine Tung; Cecilia M Dorfling; Elizabeth J van Rensburg; Linda Steele; Susan L Neuhausen; Yuan Chun Ding; Bent Ejlertsen; Anne-Marie Gerdes; Thomas v O Hansen; Teresa Ramón y Cajal; Ana Osorio; Javier Benitez; Javier Godino; Maria-Isabel Tejada; Mercedes Duran; Jeffrey N Weitzel; Kristie A Bobolis; Sharon R Sand; Annette Fontaine; Antonella Savarese; Barbara Pasini; Bernard Peissel; Bernardo Bonanni; Daniela Zaffaroni; Francesca Vignolo-Lutati; Giulietta Scuvera; Giuseppe Giannini; Loris Bernard; Maurizio Genuardi; Paolo Radice; Riccardo Dolcetti; Siranoush Manoukian; Valeria Pensotti; Viviana Gismondi; Drakoulis Yannoukakos; Florentia Fostira; Judy Garber; Diana Torres; Muhammad Usman Rashid; Ute Hamann; Susan Peock; Debra Frost; Radka Platte; D Gareth Evans; Rosalind Eeles; Rosemarie Davidson; Diana Eccles; Trevor Cole; Jackie Cook; Carole Brewer; Shirley Hodgson; Patrick J Morrison; Lisa Walker; Mary E Porteous; M John Kennedy; Louise Izatt; Julian Adlard; Alan Donaldson; Steve Ellis; Priyanka Sharma; Rita Katharina Schmutzler; Barbara Wappenschmidt; Alexandra Becker; Kerstin Rhiem; Eric Hahnen; Christoph Engel; Alfons Meindl; Stefanie Engert; Nina Ditsch; Norbert Arnold; Hans Jörg Plendl; Christoph Mundhenke; Dieter Niederacher; Markus Fleisch; Christian Sutter; C R Bartram; Nicola Dikow; Shan Wang-Gohrke; Dorothea Gadzicki; Doris Steinemann; Karin Kast; Marit Beer; Raymonda Varon-Mateeva; Andrea Gehrig; Bernhard H Weber; Dominique Stoppa-Lyonnet; Olga M Sinilnikova; Sylvie Mazoyer; Claude Houdayer; Muriel Belotti; Marion Gauthier-Villars; Francesca Damiola; Nadia Boutry-Kryza; Christine Lasset; Hagay Sobol; Jean-Philippe Peyrat; Danièle Muller; Jean-Pierre Fricker; Marie-Agnès Collonge-Rame; Isabelle Mortemousque; Catherine Nogues; Etienne Rouleau; Claudine Isaacs; Anne De Paepe; Bruce Poppe; Kathleen Claes; Kim De Leeneer; Marion Piedmonte; Gustavo Rodriguez; Katie Wakely; John Boggess; Stephanie V Blank; Jack Basil; Masoud Azodi; Kelly-Anne Phillips; Trinidad Caldes; Miguel de la Hoya; Atocha Romero; Heli Nevanlinna; Kristiina Aittomäki; Annemarie H van der Hout; Frans B L Hogervorst; Senno Verhoef; J Margriet Collée; Caroline Seynaeve; Jan C Oosterwijk; Johannes J P Gille; Juul T Wijnen; Encarna B Gómez Garcia; Carolien M Kets; Margreet G E M Ausems; Cora M Aalfs; Peter Devilee; Arjen R Mensenkamp; Ava Kwong; Edith Olah; Janos Papp; Orland Diez; Conxi Lazaro; Esther Darder; Ignacio Blanco; Mónica Salinas; Anna Jakubowska; Jan Lubinski; Jacek Gronwald; Katarzyna Jaworska-Bieniek; Katarzyna Durda; Grzegorz Sukiennicki; Tomasz Huzarski; Tomasz Byrski; Cezary Cybulski; Aleksandra Toloczko-Grabarek; Elżbieta Złowocka-Perłowska; Janusz Menkiszak; Adalgeir Arason; Rosa B Barkardottir; Jacques Simard; Rachel Laframboise; Marco Montagna; Simona Agata; Elisa Alducci; Ana Peixoto; Manuel R Teixeira; Amanda B Spurdle; Min Hyuk Lee; Sue K Park; Sung-Won Kim; Tara M Friebel; Fergus J Couch; Noralane M Lindor; Vernon S Pankratz; Lucia Guidugli; Xianshu Wang; Marc Tischkowitz; Lenka Foretova; Joseph Vijai; Kenneth Offit; Mark Robson; Rohini Rau-Murthy; Noah Kauff; Anneliese Fink-Retter; Christian F Singer; Christine Rappaport; Daphne Gschwantler-Kaulich; Georg Pfeiler; Muy-Kheng Tea; Andreas Berger; Mark H Greene; Phuong L Mai; Evgeny N Imyanitov; Amanda Ewart Toland; Leigha Senter; Anders Bojesen; Inge Sokilde Pedersen; Anne-Bine Skytte; Lone Sunde; Mads Thomassen; Sanne Traasdahl Moeller; Torben A Kruse; Uffe Birk Jensen; Maria Adelaide Caligo; Paolo Aretini; Soo-Hwang Teo; Christina G Selkirk; Peter J Hulick; Irene Andrulis Journal: JAMA Date: 2015-04-07 Impact factor: 56.272