Proteinase 3-antineutrophil cytoplasmic antibody-positive necrotizing crescentic glomerulonephritis complicated by infectious endocarditis: a case report.

BACKGROUND: Proteinase 3-antineutrophil cytoplasmic antibody has been reported to be positive in 5-10% of cases of renal injury complicated by infective endocarditis; however, histological findings have rarely been reported for these cases. CASE
PRESENTATION: A 71-year-old Japanese man with a history of aortic valve replacement developed rapidly progressive renal dysfunction with gross hematuria and proteinuria. Blood analysis showed a high proteinase 3-antineutrophil cytoplasmic antibody (163 IU/ml) titer. Streptococcus species was detected from two separate blood culture bottles. Transesophageal echocardiography detected mitral valve vegetation. Histological evaluation of renal biopsy specimens showed necrosis and cellular crescents in glomeruli without immune complex deposition. The patient met the modified Duke criteria for definitive infective endocarditis. On the basis of these findings, the patient was diagnosed with proteinase 3-antineutrophil cytoplasmic antibody-positive necrotizing crescentic glomerulonephritis complicated by Streptococcus infective endocarditis. His renal disease improved, and his proteinase 3-antineutrophil cytoplasmic antibody titer normalized with antibiotic monotherapy.
CONCLUSION: Few case reports have described histological findings of proteinase 3-antineutrophil cytoplasmic antibody-positive renal injury complicated with infective endocarditis. We believe that an accumulation of histological findings and treatments is mandatory for establishment of optimal management for proteinase 3-antineutrophil cytoplasmic antibody-positive renal injury complicated with infective endocarditis.

Background

Proteinase 3-antineutrophil cytoplasmic antibody (PR3-ANCA) has been reported to be positive in 5–10% of cases of renal injury complicated by infective endocarditis [1]; however, histological findings have rarely been reported for these cases. In addition, the clinical course and optimal treatment have not been fully clarified.

We report a case of a patient with rapidly progressive PR3-ANCA-positive necrotizing crescentic glomerulonephritis complicated by Streptococcus infective endocarditis. The patient’s renal disease improved with antibiotic therapy without any immunosuppressive agents, and his PR3-ANCA titer normalized in accordance with improving infective endocarditis.

Case presentation

Our patient was a 71-year-old Japanese man who had undergone the Bentall procedure and biological aortic valve replacement for the treatment of descending aortic aneurysm and aortic regurgitation at 70 years of age. Thereafter, his renal function had been normal (serum creatinine level, 0.93 mg/dl) without hematuria and proteinuria. Two months before admission, he had appetite loss, malaise, and gross hematuria. One month before admission, he noticed purpura on his lower extremities. A laboratory examination conducted by his primary care physician showed anemia (hemoglobin, 9.2 g/dl), thrombocytopenia (platelet count, 10 × 104/μl), hematuria, and proteinuria. Therefore, he was referred to our hospital for further management.

Upon admission, his body temperature was 36.9 °C, and his blood pressure was 120/60 mmHg. Anemia, edema, and symmetrically distributed palpable purpura of the lower extremities were observed. He had no characteristic physical findings of infective endocarditis, such as Osler nodes, Roth spots, and Janeway lesions. Cardiac auscultation revealed 2/6 systolic reflux murmur at the cardiac apex. Blood analysis showed that the patient’s serum creatinine level was elevated at 2.34 mg/dl, and his serum hemoglobin level was reduced at 7.6 g/dl. Urinalysis showed proteinuria at 0.74 g/g Cr and microscopic hematuria. PR3-ANCA level was elevated at 163 IU/ml (normal range, < 10 IU/ml). The patient had negative test results for hepatitis B antigen, hepatitis C antibody, cryoglobulin, antistreptolysin O, antinuclear antibody, immune complex, and myeloperoxidase-ANCA. Serum complement C3 was mildly decreased, whereas C4 was normal. Laboratory data obtained at admission are summarized in Table 1. No abnormalities were found in the patient’s chest x-ray or electrocardiogram. Streptococcus species was detected from two separate blood culture bottles. On the third hospital day, renal biopsy was performed. Histological analysis revealed that 54% (6 of 11) of glomeruli showed partial fibrinoid necrosis with fragmentation of glomerular tufts (Fig. 1a), and 27% (3 of 11) of glomeruli showed cellular crescents (Fig. 1b). No fibrocellular or fibrous crescents and no endocapillary proliferation were found. The mesangium showed no increase in cells or matrix. The tubulointerstitium partially showed neutrophilic and lymphocytic infiltration in the peritubular capillary and atrophy (Fig. 1c). Fibrinoid necrosis was not observed in vessel walls. Immunofluorescence microscopy showed no deposition of immunoglobulins and complement factors. Electron microscopy showed small amounts of nonspecific electron-dense deposits in subendothelial areas and the paramesangial area. At this point, the patient met the modified Duke criteria for definitive infective endocarditis [2] (mitral valve vegetation on echocardiography, two positive blood cultures of Streptococcus species drawn 3 days apart, glomerulonephritis). On the eighth hospital day, transesophageal echocardiography revealed mitral valve vegetation. On the 12th hospital day, spinal magnetic resonance imaging showed pyogenic spondylitis at T7/T8 and L4/L5. On the basis of these findings, the patient was diagnosed with rapidly progressive PR3-ANCA-positive necrotizing crescentic glomerulonephritis complicated by Streptococcus infective endocarditis. Antibiotic therapy including cefazolin and penicillin G followed by oral administration of ampicillin was provided without immunosuppressive agents. Thereafter, his renal disease, endocarditis, and pyogenic spondylitis improved. He was discharged from our center on the 73rd hospital day. He has since received regular outpatient treatment in our department. At 7 months after discharge, his serum creatinine level had decreased to 1.43 mg/dl, his proteinuria had decreased to 0.15 g/g Cr, and his hematuria had decreased to 1.1 red blood cells per high-power field. His PR3-ANCA level had decreased to within the normal range (Fig. 2).

Table 1

Laboratory findings upon admission

Complete blood count and blood chemistry	Value
WBC	13,600/μL
Bands	2%
Segments	82%
Eosinophils	0%
Basophils	0%
Lymphocytes	7%
Monocytes	8%
RBC	323 × 104/μL
Hemoglobin	7.6 g/dL
Hematocrit	29.2%
Platelet	12.0 × 104/μL
Total protein	7.0 g/dL
Albumin	2.6 g/dL
AST	35 IU/L
ALT	23 IU/L
CRP	7.57 mg/dL
Na+	130 mmol/L
K+	5.3 mmol/L
Cl−	101 mmol/L
Ca2+	7.7 mg/dL
Phosphate	3.8 mg/dL
BUN	25 mg/dL
Cr	2.52 mg/dL
eGFR	20.8 ml/minute/1.73 m2
Uric acid	6.3 mg/dL
HbA1c	6.3%
Glucose	106 mg/dL
ASO	73 IU/mL
Hepatitis B antigen	< 0.04
Hepatitis C antibody	< 0.29
IgG	2692 mg/dL
IgA	340 mg/dL
IgM	350 mg/dL
Anti-DNA antibody	< 10
Anti-RNP antibody	Negative
Anti-Sm antibody	Negative
C3	50 mg/dL
C4	17 mg/dL
CH50	23.4 U/mL
Antinuclear antibody	160
PR3-ANCA	163 IU/mL
MPO-ANCA	< 1.0 IU/mL
Anti-GBM antibody	< 2.0 IU/mL
ESR	86 mm/hour
Rheumatoid factor	86 IU/mL
Urinary analysis
RBC	Numerous (dysmorphic)/HPF
WBC	1–4/HPF
Protein	0.74 g/g Cr
β2-MG	12,133 μg/L

Abbreviations: ALT alanine aminotransferase, ASO antistreptolysin O, AST aspartate aminotransferase, β-MG β2-microglobulin, BUN blood urea nitrogen, CH50 50% homolytic unit of complement, Cr creatinine, CRP C-reactive protein, C3 complement component 3, C4 complement component 4, eGFR estimated glomerular filtration rate, ESR erythrocyte sedimentation rate, GBM antiglomerular basement membrane antibody, HbA1c hemoglobin A1c, HPF high-power field, Ig immunoglobulin, MPO-ANCA myeloperoxidase antineutrophil cytoplasmic antibody, PR3-ANCA proteinase 3 antineutrophil cytoplasmic antibody, RBC red blood cells, RNP ribonucleoprotein, Sm Smith, WBC white blood cells

Fig. 1

Renal biopsy findings. a Glomerulus with partial fibrinoid necrosis with fragmentation of glomerular tufts (arrows) (periodic acid-methenamine silver stain; original magnification, 400×). b Glomerulus with cellular crescentic formation (arrows) (periodic acid-Schiff stain; magnification, original magnification, 400×). c Tubulointerstitium with sporadic neutrophil infiltration in the peritubular capillary (arrows) and atrophy (broken line) (periodic acid-Schiff stain; original magnification, 100×)

Fig. 2

The patient’s clinical course. ABPC Ampicillin, CEZ Cefazolin, Cr Creatinine, PCG Penicillin G, PR3-ANCA Proteinase 3-antineutrophil cytoplasmic antibody

Discussion and conclusions

We report a case of rapidly progressive PR3-ANCA-positive necrotizing crescentic glomerulonephritis complicated by Streptococcus infective endocarditis. The patient’s renal disease improved with antibiotic monotherapy, which led to normalization of PR3-ANCA titer in accordance with improving infective endocarditis.

Renal disease associated with infective endocarditis shows various pathological changes including crescent formation, fibrinoid necrosis, mesangial cell proliferation, and endothelial cell thickening in the glomerulus and tubulointerstitial damage with infiltration of immune cells [3-7]. PR3-ANCA has been reported to be positive in 5–10% of cases of renal disease complicated with infective endocarditis [1]. It is considered that PR3-ANCA may be produced as a result of an immune response against infection by sharing epitopes with cytoplasmic antigens of neutrophils in cases of infective endocarditis [8]. The produced PR3-ANCA is then speculated to contribute to fibrinoid necrosis, crescent formation, and granulomas in the kidney [9]. However, the lack of sufficient histological findings of PR3-ANCA-positive renal diseases complicated by infective endocarditis prevents clarification of detailed pathological changes in the kidney. Although many cases of PR3-ANCA-positive renal disease complicated by infective endocarditis have been reported, including crescentic glomerulonephritis, endocapillary proliferative glomerulonephritis, mesangial proliferative glomerulonephritis, and focal segmental glomerulonephritis, only three cases showed necrotizing crescentic glomerulonephritis complicated by infective endocarditis [10-34] (Table 2). Regarding treatment for PR3-ANCA-positive renal disease complicated by infective endocarditis, previous studies suggested antibiotic monotherapy for patients with low PR3-ANCA titers (< 25 IU/ml) and combination therapy with immunosuppressive agents, including steroids for patients with high PR3-ANCA titers (> 50 IU/ml), when the patients’ condition does not improve with antibiotic monotherapy [22, 35]. The three previous cases of PR3-ANCA-positive necrotizing crescentic glomerulonephritis showed various PR3-ANCA titers (2.96, > 8.0, and 85 IU/ml) and were treated with immunosuppressive agents such as corticosteroids in addition to antibiotics. Among those three cases, the renal disease resolved completely in two patients but progressed to end-stage renal disease in the other (Table 2). The other types of PR3-ANCA-positive renal disease complicated by infective endocarditis also showed various PR3-ANCA titers (3–359 IU/ml) and were treated with antibiotics with or without immunosuppressive agents (Table 2). Regarding treatment outcomes, most renal diseases recovered, except for one patient with crescentic glomerulonephritis with high PR3-ANCA titers (247 IU/ml) and one patient with mesangial proliferative glomerulonephritis with high PR3-ANCA titers (143 IU/ml), both of whom died (Table 2). In our patient, necrotizing crescentic glomerulonephritis improved with antibiotic monotherapy, and PR3-ANCA titer normalized in accordance with improving infective endocarditis; however, PR3-ANCA titer was highly elevated at 163 IU/ml. The results of our patient’s case suggest that antibiotic monotherapy can be effective even if the PR3-ANCA titer is considerably high in PR3-ANCA-positive necrotizing crescentic glomerulonephritis complicated by infective endocarditis. However, caution is needed with the use of immunosuppressive agents because they may exacerbate bacteremia and infective endocarditis. Furthermore, a greater accumulation of cases with histological evidence is needed to investigate optimal treatments for PR3-ANCA-positive renal disease complicated with infective endocarditis.

Table 2

Case reports of PR3-ANCA-positive renal injury complicated by infective endocarditis

Age (years)/sex	Renal biopsy histology (IF/EM)	PR3-ANCA (IU/mL)	Microbe detected	Past medical history	Treatments	Outcome	Reference
54/M	Focal necrotizing crescentic GN (negative/no deposits)	2.96	Streptococcus mutans	MVP	Piperacillin, tazobactam, cyclophosphamide, corticosteroids	Complete recovery	[10]
59/M	Focal necrotizing crescentic GN (negative/no deposits)	> 8.0	Enterococcus faecalis	Hepatitis B and C	Pulse methylprednisolone ⇒ prednisolone	Hemodialysis	[11]
67/M	Focal necrotizing crescentic GN (IgA+, IgM+, IgG+, C3+, C1q+/mesangial and subendothelial dense deposits)	85	Gemella sanguinis	No mention	Ceftriaxone, gentamicin, methylprednisolone	Temporary hemodialysis ⇒ complete recovery	[12]
6/M	Crescentic GN (no mention)	Positive	Bartonella henselae	CHD	Doxycycline, rifampicin	No mention	[13]
12/F	Crescentic GN (C3+/subendothelial dense deposits)	Positive	Gemella morbillorum	Nothing	Penicillin, gentamicin, steroids	Complete recovery	[14]
14/M	Crescentic GN (no mention)	Positive	Bartonella henselae	CHD	Doxycycline	No mention	[13]
18/F	Crescentic GN (IgG[1+], IgM[3+], C3[2+], C1q[2+]/subendothelial dense deposits)	32	Bartonella henselae	Tetralogy of Fallot, SSS, and complete heart block	Antibiotic therapy, methylprednisolone	Complete recovery	[15]
24/M	Crescentic GN (C3+/mesangial and subendothelial dense deposits)	14	α-Hemolytic Streptococcus	VSD	Cefotaxime, prednisolone	No renal dysfunction	[16]
26/F	Crescentic GN (IgM+ C3+/no mention)	160	Streptococcus viridans	ASD, recent dental Tx	Penicillin G, tobramycin	Recovery	[17]
36/M	Crescentic GN (negative/no mention)	359	Bartonella henselae	No mention	Prednisolone, intravenous cyclophosphamide ⇒ azathioprine, MMF, prednisolone, CV surgery	Complete recovery	[18]
43/M	Crescentic GN (negative/no mention)	Positive	Bartonella henselae	Infective endocarditis (blood culture was negative)	Cyclophosphamide, prednisoloneCV surgery	No mention	[19]
46/M	Crescentic GN (C3+ C1q+/no mention)	25	Negative	No mention	Amoxicillin, gentamicin, penicillin	Complete recovery	[20]
47/M	Crescentic GN (IgM+, IgA+, C3+, C1q+/mesangial and subendothelial dense deposits)	160	Bartonella henselae	Cat scratch disease	Doxycycline, rifampicin 6 weeks	Recovery	[21]
50/M	Crescentic GN (negative/not performed)	247	Streptococcus oralis	Nothing	Steroid therapy ⇒ ampicillin, gentamicin, vancomycin	Death	[22]
54/M	Crescentic GN (IgM+, C3+/no deposits)	3	Streptococcus mutans	No mention	Ampicillin ⇒ vancomycin, corticosteroids, cyclophosphamide	Recovery	[23]
55/M	Crescentic GN (C3[2+], IgA+/no deposits)	> 8.0	Bartonella henselae, Bartonella quintana	Depression	Vancomycin, cefepime ⇒ doxycycline, rifampicin, methylprednisolone	Recovery	[24]
67/M	Crescentic GN (IgM+, C3+, C1q+/no mention)	41	Bartonella henselae	Thoracic aortic aneurysm repair	Rifampicin, doxycycline, methylprednisolone	Temporary hemodialysis ⇒ recovery	[25]
72/F	Crescentic GN (no mention)	Positive	Aggregatibacter aphrophilus	No mention	Vancomycin, ceftriaxone	Temporary hemodialysis ⇒ recovery	[26]
42/M	Diffuse endocapillary proliferative GN (C3+/subendothelial dense deposits)	21.3	Staphylococcus aureus	Nothing	Cefazolin	Recovery	[27]
68/M	Diffuse endocapillary proliferative GN and crescentic GN (IgG[2+], IgM[3+], C3[3+], C1q[2+]/subendothelial dense deposits)	102	Negative	Schistosomiasis	Cefoperazone, tazobactam	Complete recovery	[28]
78/F	Endocapillary proliferative GN (IgM+, C3+, C1q+/no mention)	30	Bartonella henselae	Hypertension	Doxycycline	Complete recovery	[29]
48/M	Mesangial proliferative GN (C3+/no mention)	12	Negative	Alcoholism, DM	Amoxicillin, gentamicin	Complete recovery	[20]
57/M	Mesangial proliferative GN (IgG+, IgM+, C3+ /no mention)	45	Negative	Nothing	Corticosteroids ⇒ ampicillin, ceftriaxone, gentamicin, vancomycin	Recovery	[30]
74/M	Mesangial proliferative GN (IgG+, C1q+/not performed)	> 100	Bartonella henselae, Bartonella quintana	IHD, pacemaker, DM, pulmonary embolus	Antibiotic therapy	Recovery	[31]
78/M	Mesangial proliferative GN (IgM+, C3+, IgA+, C1q+/subendothelial dense deposits)	143	Enterococcus faecalis	Coronary artery bypass surgery	Antibiotic therapy	Death	[32]
57/M	FSGS (IgM+, C3+/paramesangial dense deposits)	40	Negative	DM, AVR, aortic aneurysm	Pulse methylprednisolone ⇒ vancomycin, gentamicin, rifampicin	Plasmapheresis ⇒ recovery	[33]
64/M	FSGS and mild interstitial inflammation (no mention)	60	Negative	Insidious mild renal dysfunction	Ceftriaxone, doxycycline	Complete recovery	[34]

Abbreviations: ASD atrial septal defect, AVR aortic valve replacement, CHD chronic heart disease, C3 complement component 3, CV cardiovascular, DM diabetes mellitus, EM electron microscopy, F female, FSGS focal segmental glomerulonephritis, GN glomerular nephritis, IF immunofluorescence, IHD ischemic heart disease, Ig immunoglobulin, LM light microscopy, M male, MMF mycophenolate mofetil, MVP mitral valve prolapse, PR3-ANCA proteinase 3 antineutrophil cytoplasmic antibody, SSS sick sinus syndrome, Tx treatment, VSD ventricular septal defect

In conclusion, we describe a case of a patient with PR3-ANCA-positive necrotizing crescentic glomerulonephritis complicated by infective endocarditis. His renal disease was improved with antibiotic agents, and his PR3-ANCA titer normalized in accordance with improving infective endocarditis.