Yingying Wu1,2, Xin Wang3,4,5, Shuaishuai Liu1, Hao Luo1,6, Qiang Lin1,2. 1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, People's Republic of China. 2. University of the Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. 3. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, 510301, People's Republic of China. wangxin313@mails.ucas.ac.cn. 4. Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, People's Republic of China. wangxin313@mails.ucas.ac.cn. 5. University of the Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. wangxin313@mails.ucas.ac.cn. 6. College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, People's Republic of China.
Abstract
BACKGROUND: The evolution of male pregnancy is the most distinctive characteristic of syngnathids, and they were recognized as flagship species for marine conservation. Genus Halicampus is an important branch of syngnathid fishes that has not received the attention it deserves. OBJECTIVE: To sequence the mitochondrial genome of Halicampus grayi, and investage the genetic structure of its populations. METHODS: Degenerate primers were designed to amplify the entire mitochondrial genome of H. grayi. The phylogenetic relationship between H. grayi and other syngnathids were conducted using maximum-likelihood method. Population genetic structure of three geographic population of H. grayi were determined using median-joining haplotype network based on COI and Cytb sequences. RESULTS: The complete mitochondrial genome of Halicampus grayi was assembled into a 17,059 bp circular sequence, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 D-loop region. The overall base composition of H. grayi is 29.93% A, 29.31% T, 16.23% G and 24.54% C, with a slight A + T rich feature (59.24%). Phylogenetic analysis indicated that H. grayi has a close relationship with Trachyrhamphus serratus. Population genetic analysis revealed a relatively high genetic diversity across different geographic populations of H. grayi, and the results of median-joining haplotype network indicated a lack of structure in populations of H. grayi. CONCLUSION: The mitogenome of H. grayi will provided important information about the origin and evolution issues of syngnathid fishes, and the high-level genetic diversity detected in their populations will provide insight into the gene flow pattern of marine fishes.
BACKGROUND: The evolution of male pregnancy is the most distinctive characteristic of syngnathids, and they were recognized as flagship species for marine conservation. Genus Halicampus is an important branch of syngnathid fishes that has not received the attention it deserves. OBJECTIVE: To sequence the mitochondrial genome of Halicampus grayi, and investage the genetic structure of its populations. METHODS: Degenerate primers were designed to amplify the entire mitochondrial genome of H. grayi. The phylogenetic relationship between H. grayi and other syngnathids were conducted using maximum-likelihood method. Population genetic structure of three geographic population of H. grayi were determined using median-joining haplotype network based on COI and Cytb sequences. RESULTS: The complete mitochondrial genome of Halicampus grayi was assembled into a 17,059 bp circular sequence, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 D-loop region. The overall base composition of H. grayi is 29.93% A, 29.31% T, 16.23% G and 24.54% C, with a slight A + T rich feature (59.24%). Phylogenetic analysis indicated that H. grayi has a close relationship with Trachyrhamphus serratus. Population genetic analysis revealed a relatively high genetic diversity across different geographic populations of H. grayi, and the results of median-joining haplotype network indicated a lack of structure in populations of H. grayi. CONCLUSION: The mitogenome of H. grayi will provided important information about the origin and evolution issues of syngnathid fishes, and the high-level genetic diversity detected in their populations will provide insight into the gene flow pattern of marine fishes.