Jinxin Xie1, Panpan Tong1, Aoyuntuya Zhang1, Yongtao Yan1, Lei Zhang1, Xiaozhen Song1, Jianxing Chen1, Shaohua Zhai1, Nuerlan Shaya2, Dechao Wang1, Zhanqiang Su3, Ling Kuang4. 1. College of Veterinary Medicine, Xinjiang Agricultural University, Wulumuqi, 830052, Xinjiang Province, People's Republic of China. 2. Yili Center of Animal Disease Control and Diagnosis, Yili Animal Health Inspection, Yining, 835000, Xinjiang Province, People's Republic of China. 3. College of Veterinary Medicine, Xinjiang Agricultural University, Wulumuqi, 830052, Xinjiang Province, People's Republic of China. szq00009@163.com. 4. College of Veterinary Medicine, Xinjiang Agricultural University, Wulumuqi, 830052, Xinjiang Province, People's Republic of China. kuangling62@126.com.
Abstract
BACKGROUND: In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cause of horse death, using virome. RESULTS: We have identified an Equ1-like virus from the fecal virome of a dead thoroughbred mare in China. Full genomic sequencing and phylogenetic analysis of the virus, tentatively named "kirkovirus Cj-7-7", showed that it was closely related to kirkovirus Equ1 and clustered together with po-circo-like viruses 21, 22, 41, and 51, suggesting that it should be assigned to the proposed family "Kirkoviridae". An epidemiological investigation showed that kirkovirus Cj-7-7 circulates in horses of northern Xinjiang and may specifically infect intestinal cells. CONCLUSIONS: Our findings demonstrate the genetic diversity and geographic distribution of Kirkoviruses, and the prevalence of Kirkovirus Cj-7-7 in Xinjiang, China.
BACKGROUND: In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cause of horsedeath, using virome. RESULTS: We have identified an Equ1-like virus from the fecal virome of a dead thoroughbred mare in China. Full genomic sequencing and phylogenetic analysis of the virus, tentatively named "kirkovirus Cj-7-7", showed that it was closely related to kirkovirus Equ1 and clustered together with po-circo-like viruses 21, 22, 41, and 51, suggesting that it should be assigned to the proposed family "Kirkoviridae". An epidemiological investigation showed that kirkovirus Cj-7-7 circulates in horses of northern Xinjiang and may specifically infect intestinal cells. CONCLUSIONS: Our findings demonstrate the genetic diversity and geographic distribution of Kirkoviruses, and the prevalence of Kirkovirus Cj-7-7 in Xinjiang, China.
Entities:
Keywords:
Dead horse; Genotic diversity; North Xinjiang; Novel kirkovirus