Literature DB >> 31792826

Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells.

Meghan Cansler1, Megan Null1, Erin Meermeier2, Gwendolyn Swarbrick1, David Lewinsohn1,2,3, Melanie Harriff4,5.   

Abstract

Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.

Entities:  

Keywords:  MAIT cells; MR1-restricted T cells; T cell cloning

Mesh：

Substances：

Year:  2020        PMID： 31792826      PMCID： PMC7063788          DOI： 10.1007/978-1-0716-0207-2_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  Diversity of T Cells Restricted by the MHC Class I-Related Molecule MR1 Facilitates Differential Antigen Recognition.

Authors:  Nicholas A Gherardin; Andrew N Keller; Rachel E Woolley; Jérôme Le Nours; David S Ritchie; Paul J Neeson; Richard W Birkinshaw; Sidonia B G Eckle; John N Waddington; Ligong Liu; David P Fairlie; Adam P Uldrich; Daniel G Pellicci; James McCluskey; Dale I Godfrey; Jamie Rossjohn
Journal:  Immunity       Date:  2016-01-12       Impact factor: 31.745

2.  High expression of CD26 accurately identifies human bacteria-reactive MR1-restricted MAIT cells.

Authors:  Prabhat K Sharma; Emily B Wong; Ruth J Napier; William R Bishai; Thumbi Ndung'u; Victoria O Kasprowicz; Deborah A Lewinsohn; David M Lewinsohn; Marielle C Gold
Journal:  Immunology       Date:  2015-03-29       Impact factor: 7.397

3.  Antimicrobial activity of mucosal-associated invariant T cells.

Authors:  Lionel Le Bourhis; Emmanuel Martin; Isabelle Péguillet; Amélie Guihot; Nathalie Froux; Maxime Coré; Eva Lévy; Mathilde Dusseaux; Vanina Meyssonnier; Virginie Premel; Charlotte Ngo; Béatrice Riteau; Livine Duban; Delphine Robert; Shouxiong Huang; Martin Rottman; Claire Soudais; Olivier Lantz
Journal:  Nat Immunol       Date:  2010-06-27       Impact factor: 25.606

4.  Human mucosal associated invariant T cells detect bacterially infected cells.

Authors:  Marielle C Gold; Stefania Cerri; Susan Smyk-Pearson; Meghan E Cansler; Todd M Vogt; Jacob Delepine; Ervina Winata; Gwendolyn M Swarbrick; Wei-Jen Chua; Yik Y L Yu; Olivier Lantz; Matthew S Cook; Megan D Null; David B Jacoby; Melanie J Harriff; Deborah A Lewinsohn; Ted H Hansen; David M Lewinsohn
Journal:  PLoS Biol       Date:  2010-06-29       Impact factor: 8.029

5.  In vivo migration and function of transferred HIV-1-specific cytotoxic T cells.

Authors:  S J Brodie; D A Lewinsohn; B K Patterson; D Jiyamapa; J Krieger; L Corey; P D Greenberg; S R Riddell
Journal:  Nat Med       Date:  1999-01       Impact factor: 53.440

6.  T-cell activation by transitory neo-antigens derived from distinct microbial pathways.

Authors:  Alexandra J Corbett; Sidonia B G Eckle; Richard W Birkinshaw; Ligong Liu; Onisha Patel; Jennifer Mahony; Zhenjun Chen; Rangsima Reantragoon; Bronwyn Meehan; Hanwei Cao; Nicholas A Williamson; Richard A Strugnell; Douwe Van Sinderen; Jeffrey Y W Mak; David P Fairlie; Lars Kjer-Nielsen; Jamie Rossjohn; James McCluskey
Journal:  Nature       Date:  2014-04-02       Impact factor: 49.962

7.  Parallel T-cell cloning and deep sequencing of human MAIT cells reveal stable oligoclonal TCRβ repertoire.

Authors:  Marco Lepore; Artem Kalinichenko; Artem Kalinicenko; Alessia Colone; Bhairav Paleja; Amit Singhal; Andreas Tschumi; Bernett Lee; Michael Poidinger; Francesca Zolezzi; Luca Quagliata; Peter Sander; Evan Newell; Antonio Bertoletti; Luigi Terracciano; Gennaro De Libero; Lucia Mori
Journal:  Nat Commun       Date:  2014-05-15       Impact factor: 14.919

8.  MR1-restricted MAIT cells display ligand discrimination and pathogen selectivity through distinct T cell receptor usage.

Authors:  Marielle C Gold; James E McLaren; Joseph A Reistetter; Sue Smyk-Pearson; Kristin Ladell; Gwendolyn M Swarbrick; Yik Y L Yu; Ted H Hansen; Ole Lund; Morten Nielsen; Bram Gerritsen; Can Kesmir; John J Miles; Deborah A Lewinsohn; David A Price; David M Lewinsohn
Journal:  J Exp Med       Date:  2014-07-21       Impact factor: 14.307

9.  Antigen-loaded MR1 tetramers define T cell receptor heterogeneity in mucosal-associated invariant T cells.

Authors:  Rangsima Reantragoon; Alexandra J Corbett; Isaac G Sakala; Nicholas A Gherardin; John B Furness; Zhenjun Chen; Sidonia B G Eckle; Adam P Uldrich; Richard W Birkinshaw; Onisha Patel; Lyudmila Kostenko; Bronwyn Meehan; Katherine Kedzierska; Ligong Liu; David P Fairlie; Ted H Hansen; Dale I Godfrey; Jamie Rossjohn; James McCluskey; Lars Kjer-Nielsen
Journal:  J Exp Med       Date:  2013-10-07       Impact factor: 14.307

10.  Human TRAV1-2-negative MR1-restricted T cells detect S. pyogenes and alternatives to MAIT riboflavin-based antigens.

Authors:  Erin W Meermeier; Bruno F Laugel; Andrew K Sewell; Alexandra J Corbett; Jamie Rossjohn; James McCluskey; Melanie J Harriff; Tamera Franks; Marielle C Gold; David M Lewinsohn
Journal:  Nat Commun       Date:  2016-08-16       Impact factor: 14.919
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