Kazuyoshi Hirota1, Tetsuya Kushikata2, Daiki Takekawa2, Mihoko Kudo2. 1. Department of Anesthesiology, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Japan. hirotak@hirosaki-u.ac.jp. 2. Department of Anesthesiology, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Japan.
Abstract
INTRODUCTION: It is believed that neurotransmitters release modulates general anesthesia via several receptors system which are molecular targets for anesthetic agents in young-adult rats. However, middle-aged rats have rarely been used. Therefore, we studied in this age group. MATERIALS AND METHODS: After approval of our protocol by the institutional committee on animal research, 116 middle aged Sprague-Dawley rats were assigned to ketamine (K: n = 74) and propofol (P: n = 42) anesthesia groups. Rats were decapitated 0, 20 60 and 120 min after ip K (100 mg/kg) or P (80 mg/kg), respectively. Melanin-concentrating hormone (MCH), orexin A (OXA) and noradrenaline contents in the pons, hypothalamus, hippocampus and cerebrocortex were measured by a commercial enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography. RESULTS: Neurotransmitter content in all brain regions did not significantly change following K or P administration. CONCLUSION: Therefore, we question whether neurotransmitter release contributes to general anesthesia.
INTRODUCTION: It is believed that neurotransmitters release modulates general anesthesia via several receptors system which are molecular targets for anesthetic agents in young-adult rats. However, middle-aged rats have rarely been used. Therefore, we studied in this age group. MATERIALS AND METHODS: After approval of our protocol by the institutional committee on animal research, 116 middle aged Sprague-Dawley rats were assigned to ketamine (K: n = 74) and propofol (P: n = 42) anesthesia groups. Rats were decapitated 0, 20 60 and 120 min after ip K (100 mg/kg) or P (80 mg/kg), respectively. Melanin-concentrating hormone (MCH), orexin A (OXA) and noradrenaline contents in the pons, hypothalamus, hippocampus and cerebrocortex were measured by a commercial enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography. RESULTS: Neurotransmitter content in all brain regions did not significantly change following K or P administration. CONCLUSION: Therefore, we question whether neurotransmitter release contributes to general anesthesia.
Entities:
Keywords:
Aging; Mechanism of general anesthesia; Neurotransmitter release; Rats
Authors: Andrew H Song; Aaron Kucyi; Vitaly Napadow; Emery N Brown; Marco L Loggia; Oluwaseun Akeju Journal: J Neurosci Date: 2017-06-16 Impact factor: 6.167