Shuaihua Li1,2, Qiyu Tang1,3, Hongbo Xu4, Qiangru Huang1, Zi Wen1, Yawei Liu1, Cheng Peng1. 1. Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China. 2. Department of Cosmetic & Plastic Surgery, The First People's Hospital of Chenzhou, Chenzhou 423000, Hunan, PR China. 3. Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, PR China. 4. Department of General Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
Abstract
Aim: Diabetic wound healing is seriously interrupted, and administration of KGF for wound treatment is restricted by its inherent instability. We aim to develop an ideal way toward KGF stabilization, thus improving diabetic wound healing. Materials & methods: We conjugated KGF with gold nanoparticles (GNPs) and determined the stability and binding affinity. Biological effects of conjugates (KGF-GNPs) were evaluated in vitro and in an animal model. Results: KGF-GNPs revealed high stability under hostile circumstances because of the preserved secondary structure and possessed elevated binding affinity to KGF receptor. Moreover, application of KGF-GNPs contributed to accelerated wound recovery in diabetic rats, including re-epithelialization and contraction. Conclusion: KGF-GNPs were promising for future clinical application for diabetic wound therapy.
Aim: Diabetic wound healing is seriously interrupted, and administration of KGF for wound treatment is restricted by its inherent instability. We aim to develop an ideal way toward KGF stabilization, thus improving diabetic wound healing. Materials & methods: We conjugated KGF with gold nanoparticles (GNPs) and determined the stability and binding affinity. Biological effects of conjugates (KGF-GNPs) were evaluated in vitro and in an animal model. Results:KGF-GNPs revealed high stability under hostile circumstances because of the preserved secondary structure and possessed elevated binding affinity to KGF receptor. Moreover, application of KGF-GNPs contributed to accelerated wound recovery in diabeticrats, including re-epithelialization and contraction. Conclusion:KGF-GNPs were promising for future clinical application for diabetic wound therapy.
Authors: Beyza Bulutoglu; Aylin Acun; Sarah L Deng; Safak Mert; Elise Lupon; Alexandre G Lellouch; Curtis L Cetrulo; Basak E Uygun; Martin L Yarmush Journal: Adv Healthc Mater Date: 2022-04-28 Impact factor: 11.092