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Literature DB >> 3178730

Comparative 113Cd-n.m.r. studies on rabbit 113Cd7-, (Zn1,Cd6)- and partially metal-depleted 113Cd6-metallothionein-2a.

Abstract

Rabbit 113Cd7-metallothionein-2a (MT) contains two metal-thiolate clusters of three (cluster B) and four (cluster A) metal ions. The 113Cd-n.m.r. spectrum of 113Cd6-MT, isolated from 113Cd7-MT upon treatment with EDTA, is similar to that of 113Cd7-MT, but the cluster B resonances are lower in intensity, suggesting its co-operative metal depletion. (Zn1,113Cd6)-MT, formed upon addition of the Zn(II) ions to 113Cd6-MT, shows 113Cd-n.m.r. features characteristic of cluster B populations containing both Cd(II) and Zn(II) ions. The overall intensity gain of the mixed cluster B resonances per Cd as to those in 113Cd6- and 113Cd7-MT suggests a stabilization effect of the bound Zn(II) ions upon the previously established intramolecular 113Cd exchange within this cluster.

Entities: Chemical Gene Species

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Year: 1988 PMID： 3178730 PMCID： PMC1149342 DOI： 10.1042/bj2530611

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

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Authors: M Kimura; N Otaki; M Imano
Journal: Experientia Suppl Date: 1979

2. Equine hepatic and renal metallothioneins. Purification, molecular weight, amino acid composition, and metal content.

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Journal: J Biol Chem Date: 1974-06-10 Impact factor: 5.157

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Authors: R H Bühler; J H Kägi
Journal: Experientia Suppl Date: 1979

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Authors: K B Nielson; D R Winge
Journal: J Biol Chem Date: 1983-11-10 Impact factor: 5.157

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Authors: M Vasák; E Wörgötter; G Wagner; J H Kägi; K Wüthrich
Journal: J Mol Biol Date: 1987-08-05 Impact factor: 5.469

6. 113Cd NMR studies of reconstituted seven-cadmium metallothionein: evidence for structural flexibility.

Authors: M Vasák; G E Hawkes; J K Nicholson; P J Sadler
Journal: Biochemistry Date: 1985-01-29 Impact factor: 3.162

7. Metal thiolate clusters in cobalt(II)-metallothionein.

Authors: M Vasák; J H Kägi
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

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Authors: J D Otvos; I M Armitage
Journal: Proc Natl Acad Sci U S A Date: 1980-12 Impact factor: 11.205

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Authors: D R Winge; K A Miklossy
Journal: J Biol Chem Date: 1982-04-10 Impact factor: 5.157

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Authors: J D Otvos; H R Engeseth; S Wehrli
Journal: Biochemistry Date: 1985-11-19 Impact factor: 3.162

6 in total

6. Monitoring metal ion flux in reactions of metallothionein and drug-modified metallothionein by electrospray mass spectrometry.

Authors: J Zaia; D Fabris; D Wei; R L Karpel; C Fenselau
Journal: Protein Sci Date: 1998-11 Impact factor: 6.725