Volume and length changes in outer hair cells of the guinea pig after potassium-induced shortening.

Measurements of volume and length were made on isolated guinea pig cochlear outer hair cells (OHCs) in an attempt to establish whether OHC shortening was accompanied by changes in cell volume. A sustained shortening in response to an isotonic 100 mM KCl solution was found to be accompanied by a significant increase in OHC volume. The application of hypotonic solutions had a similar effect. When testing solutions with unchanged or reduced [K+] [Cl-] products in order to avoid loading the cells with chloride ions and producing concomitant water influx, the most frequent response was a rapid shortening followed by an elongation beyond the original cell length. These findings of a sustained and a spontaneously reversible shortening suggest that the potassium-induced response may consist of two components: a rapid one, which may be reversible in the presence of the stimulus, and a second, slower, component resulting in sustained shortening.