Wenhao Qian1, Jiajun Qiu2,3, Xuanyong Liu2,3. 1. Shanghai Xuhui District Dental Center, Shanghai, China. 2. State Key Laboratory of High-Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China. 3. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, China.
Abstract
BACKGROUND: Dental implant failures still occur because of peri-implant diseases such as peri-implantitis. The objective of this study was to treat peri-implantitis in beagle dogs by fabricating minocycline hydrochloride (MH)-loaded graphene oxide (GO) films on implant abutment surfaces. METHODS: Beagle dogs with silk ligature were used to establish the peri-implantitis model. Modified sulcus bleeding index, peri-implant probing pocket depth, radiographic evaluation, micro-CT tomography analysis, and histological evaluation were determined to evaluate the therapeutic effect of MH-loaded GO films on abutment surfaces for peri-implantitis. RESULTS: Radiographic and micro-CT analysis showed that lots of marginal bone loss could be found on Ti and MH/Ti groups, especially for Ti group. Little bone less could be seen on GO/Ti group while bone less on MH/GO/Ti group was negligible. Results of the histological analysis presented that lots of neutrophils could be found on Ti and MH/Ti groups. However, almost none of the neutrophils could be observed on GO/Ti and MH/GO/Ti while lots of osteocytes could be found. CONCLUSION: MH-loaded GO films on implant abutment surfaces could prevent the further development of peri-implantitis and exhibit good therapeutic effect for peri-implantitis in beagle dogs.
BACKGROUND: Dental implant failures still occur because of peri-implant diseases such as peri-implantitis. The objective of this study was to treat peri-implantitis in beagle dogs by fabricating minocycline hydrochloride (MH)-loaded graphene oxide (GO) films on implant abutment surfaces. METHODS:Beagle dogs with silk ligature were used to establish the peri-implantitis model. Modified sulcus bleeding index, peri-implant probing pocket depth, radiographic evaluation, micro-CT tomography analysis, and histological evaluation were determined to evaluate the therapeutic effect of MH-loaded GO films on abutment surfaces for peri-implantitis. RESULTS: Radiographic and micro-CT analysis showed that lots of marginal bone loss could be found on Ti and MH/Ti groups, especially for Ti group. Little bone less could be seen on GO/Ti group while bone less on MH/GO/Ti group was negligible. Results of the histological analysis presented that lots of neutrophils could be found on Ti and MH/Ti groups. However, almost none of the neutrophils could be observed on GO/Ti and MH/GO/Ti while lots of osteocytes could be found. CONCLUSION:MH-loaded GO films on implant abutment surfaces could prevent the further development of peri-implantitis and exhibit good therapeutic effect for peri-implantitis in beagle dogs.
Authors: R Di Raimondo; Javier Sanz-Esporrin; I Sanz Martin; F Vignoletti; J Nuñez; F Muñoz; H J Haugen; M Sanz Journal: Clin Oral Investig Date: 2021-01-07 Impact factor: 3.573