Ming Li1,2,3, Haijuan Xie1,2,3, Miaomiao He1,2,3, Wang Su1,2,3, Yongzhi Yang1,2,3, Jian Wang1,2,3, Guangji Ye4,5,6, Yun Zhou7,8,9. 1. National Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, Qinghai, China. 2. Key Laboratory of Qinghai-Tibet Plateau Biotechnology Ministry of Education, Xining, 810016, Qinghai, China. 3. Qinghai Provincial Key Laboratory of Potato Breeding, Xining, 810016, Qinghai, China. 4. National Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, Qinghai, China. 13997089595@163.com. 5. Key Laboratory of Qinghai-Tibet Plateau Biotechnology Ministry of Education, Xining, 810016, Qinghai, China. 13997089595@163.com. 6. Qinghai Provincial Key Laboratory of Potato Breeding, Xining, 810016, Qinghai, China. 13997089595@163.com. 7. National Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, Qinghai, China. zhouyun75@163.com. 8. Key Laboratory of Qinghai-Tibet Plateau Biotechnology Ministry of Education, Xining, 810016, Qinghai, China. zhouyun75@163.com. 9. Qinghai Provincial Key Laboratory of Potato Breeding, Xining, 810016, Qinghai, China. zhouyun75@163.com.
Abstract
BACKGROUND: The sugar will eventually be exported transporter (SWEET) family is a novel type of membrane-embedded sugar transporter that contains seven transmembrane helices with two MtN3/saliva domains. The SWEET family plays crucial roles in multiple processes, including carbohydrate transportation, development, environmental adaptability and host-pathogen interactions. Although SWEET genes, especially those involved in response to biotic stresses, have been extensively characterized in many plants, they have not yet been studied in potato. OBJECTIVE: The identification of StSWEET genes provides important candidates for further functional analysis and lays the foundation for the production of good quality and high yield potatoes through molecular breeding. METHODS: In this study, StSWEET genes were identified using a genome-wide search method. A comprehensive analysis of StSWEET family through bioinformatics methods, such as phylogenetic tree, gene structure and promoter prediction analysis. The expression profiles of StSWEET genes in different potato tissues and under P. infestans attack and sugar stress were studied using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Phylogenetic analysis classified 33 StSWEET genes into four groups containing 12, 5, 12 and 4 genes. Furthermore, the gene structures and conserved motifs found that the StSWEET genes are very conservative during evolution. The chromosomal localization pattern showed that the distribution and density of the StSWEETs on 10 potato chromosomes were uneven and basically clustered. Predictive promoter analysis indicated that StSWEET proteins are associated with cell growth, development, secondary metabolism, and response to biotic and abiotic stresses. Finally, the expression patterns of the StSWEET genes in different tissues and the induction of P. infestans and the process of the sugar stress were investigated to obtain the tissue-specific and stress-responsive candidates. CONCLUSION: This study systematically identifies the SWEET gene family in potato at the genome-wide level, providing important candidates for further functional analysis and contributing to a better understanding of the molecular basis of development and tolerance in potato.
BACKGROUND: The sugar will eventually be exported transporter (SWEET) family is a novel type of membrane-embedded sugar transporter that contains seven transmembrane helices with two MtN3/saliva domains. The SWEET family plays crucial roles in multiple processes, including carbohydrate transportation, development, environmental adaptability and host-pathogen interactions. Although SWEET genes, especially those involved in response to biotic stresses, have been extensively characterized in many plants, they have not yet been studied in potato. OBJECTIVE: The identification of StSWEET genes provides important candidates for further functional analysis and lays the foundation for the production of good quality and high yield potatoes through molecular breeding. METHODS: In this study, StSWEET genes were identified using a genome-wide search method. A comprehensive analysis of StSWEET family through bioinformatics methods, such as phylogenetic tree, gene structure and promoter prediction analysis. The expression profiles of StSWEET genes in different potato tissues and under P. infestans attack and sugar stress were studied using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Phylogenetic analysis classified 33 StSWEET genes into four groups containing 12, 5, 12 and 4 genes. Furthermore, the gene structures and conserved motifs found that the StSWEET genes are very conservative during evolution. The chromosomal localization pattern showed that the distribution and density of the StSWEETs on 10 potato chromosomes were uneven and basically clustered. Predictive promoter analysis indicated that StSWEET proteins are associated with cell growth, development, secondary metabolism, and response to biotic and abiotic stresses. Finally, the expression patterns of the StSWEET genes in different tissues and the induction of P. infestans and the process of the sugar stress were investigated to obtain the tissue-specific and stress-responsive candidates. CONCLUSION: This study systematically identifies the SWEET gene family in potato at the genome-wide level, providing important candidates for further functional analysis and contributing to a better understanding of the molecular basis of development and tolerance in potato.
Authors: Edwin A G van der Vossen; Jack Gros; Anne Sikkema; Marielle Muskens; Doret Wouters; Petra Wolters; Andy Pereira; Sjefke Allefs Journal: Plant J Date: 2005-10 Impact factor: 6.417
Authors: Paul Julian Kersey; James E Allen; Irina Armean; Sanjay Boddu; Bruce J Bolt; Denise Carvalho-Silva; Mikkel Christensen; Paul Davis; Lee J Falin; Christoph Grabmueller; Jay Humphrey; Arnaud Kerhornou; Julia Khobova; Naveen K Aranganathan; Nicholas Langridge; Ernesto Lowy; Mark D McDowall; Uma Maheswari; Michael Nuhn; Chuang Kee Ong; Bert Overduin; Michael Paulini; Helder Pedro; Emily Perry; Giulietta Spudich; Electra Tapanari; Brandon Walts; Gareth Williams; Marcela Tello-Ruiz; Joshua Stein; Sharon Wei; Doreen Ware; Daniel M Bolser; Kevin L Howe; Eugene Kulesha; Daniel Lawson; Gareth Maslen; Daniel M Staines Journal: Nucleic Acids Res Date: 2015-11-17 Impact factor: 16.971