| Literature DB >> 31782037 |
Guanxiu Xiao1,2, Junqing Du2, Huihui Wu2, Xinqi Ge1,2, Xiaofeng Xu2, Aifen Yang2, Ying Zhu3, Xuemei Hu3, Kang Zheng2,3, Qiang Zhu3, Mengsheng Qiu4,5,6.
Abstract
In the developing central nervous system, the terminal differentiation of oligodendrocytes (OLs) is regulated by both extrinsic and intrinsic factors. Recent studies have suggested that the Notch-Hes signaling pathway influences the maturation of oligodendrocytes in culture and during development. However, the specific Notch receptors and their downstream effectors Hes genes that are involved in oligodendrocyte maturation have not been investigated systematically. In this study, we showed that Notch1 and Notch3 are expressed in oligodendrocyte precursor cells (OPCs) during gliogenesis, and Hes5 is the major Notch downstream transcription factor that is transiently expressed in OPCs. Overexpression of Notch intracellular domain (NICD) and Hes5 proteins in embryonic chicken spinal cord suppressed both the endogenous and Sox10-induced Mbp gene expression. Unexpectedly, overexpression of NICD/Hes5 did not inhibit Sox10 induction of Olig2 expression and Myrf induced Mbp expression, suggesting the differential inhibitory effects of NICD/Hes5 signaling on Sox10 activation of myelin-related genes and early progenitor genes.Entities:
Keywords: Differentiation; Expression pattern; Hes5; Oligodendrocytes
Year: 2019 PMID： 31782037 DOI： 10.1007/s10571-019-00764-7
Source DB: PubMed Journal: Cell Mol Neurobiol ISSN： 0272-4340 Impact factor: 5.046