| Literature DB >> 31778688 |
Xingkui An1, Adel Khashaveh1, Danfeng Liu1, Yong Xiao1, Qi Wang1, Shanning Wang2, Ting Geng3, Shaohua Gu1, Yongjun Zhang4.
Abstract
Traps baited with female-produced sex pheromones have been very effective in the monitoring and management of mirid bugs in numerous field trials. However, none of the target odorant receptors for sex pheromone components in Apolygus lucorum have been identified. Here, we identified one candidate sex pheromone receptor, AlucOR4, from A. lucorum. Quantitative real-time PCR (qPCR) analysis revealed that AlucOR4 was antennae-enriched and male-biased in adult A. lucorum. Xenopus oocyte expression system assays demonstrated that AlucOR4/AlucOrco was sensitive to two major sex pheromone constituents and exhibited high sensitivity to (E)-2-hexenyl butyrate (E2HB) and lower sensitivity to hexyl butyrate (HB). The expression level of target mRNA was significantly reduced (>80%) in dsAlucOR4-injected bugs after five days. The electroantennogram (EAG) responses of male antennae to E2HB and HB were also reduced significantly (~40%). Our findings suggest that AlucOR4 is essential to sex pheromone perception in A. lucorum.Entities:
Keywords: AlucOR4; Apolygus lucorum; Electroantennogram (EAG); RNAi; Sex pheromone receptor; Xenopus oocyte recording
Year: 2019 PMID： 31778688 DOI： 10.1016/j.jinsphys.2019.103986
Source DB: PubMed Journal: J Insect Physiol ISSN： 0022-1910 Impact factor: 2.354