| Literature DB >> 31776934 |
Agnieszka Jozwik1,2, Alan Dunlop1,3, Katy Sanchez1,3, Reuben Benjamin4,5.
Abstract
Chimeric antigen receptor T cell (CAR-T) therapies have now entered mainstream clinical practice with two approved autologous CAR-T products targeting CD19 and numerous other products in early and late phase clinical trials. This has led to a demand for highly sensitive, specific, and easily reproducible methods to monitor CAR-T cells in patients. Here we describe a flow cytometry based protocol for detection of allogeneic CAR-T cells and for monitoring their phenotype and numbers in blood and bone marrow of patients following CAR-T treatment.Entities:
Keywords: Allogeneic; CAR-T cells; Cancer; Flow cytometry; Gene knockout; Immunotherapy
Year: 2020 PMID： 31776934 DOI： 10.1007/978-1-0716-0203-4_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745