Doris Bismarck1, Anika Dusold2,3, Anton Heusinger2, Elisabeth Müller2. 1. Laboklin GmbH & Co. KG, Bad Kissingen, Germany, bismarck@laboklin.com. 2. Laboklin GmbH & Co. KG, Bad Kissingen, Germany. 3. Coburg University of Applied Sciences and Arts, Coburg, Germany.
Abstract
BACKGROUND: The yeast Malassezia pachydermatis is a commensal but also the most isolated pathogenic yeast in canine otitis externa. Essential oils (EOs) exhibit antimicrobial activity and thus could be an alternative for treatment of otitis. OBJECTIVE: The aim of this study was to analyse the in vitro efficacy of EOs against M. pachydermatis isolates from canine ears. MATERIALS AND METHODS: Twenty-two EOs were tested for their in vitro activity against 15 M. pachydermatis isolates by agar disc diffusion. In addition, diluted EOs were tested and volatile antifungal activity was analysed by vapour assay. RESULTS: Most EOs showed in vitro efficacy against M. pachydermatis. A different susceptibility pattern of tested isolates was found. Overall, the most potential EO was lemon grass. Also, cinnamon leaf, clove, manuka, Indian melissa, oregano, palmarosa, and winter savory oil exhibited a strong antifungal activity. Volatile activity was proven by the inhibiting effect in vapour assay. CONCLUSION: EOs show in vitro activity against M. pachydermatis. This study gives scientific background for the use of EOs in veterinary medicine. Diverse susceptibility patterns of different M. pachydermatis strains emphasise the helpfulness of an aromatogram as one lead for the best choice of an EO for therapy. The Author(s). Published by S. Karger AG, Basel.
BACKGROUND: The yeastMalassezia pachydermatis is a commensal but also the most isolated pathogenic yeast in canineotitis externa. Essential oils (EOs) exhibit antimicrobial activity and thus could be an alternative for treatment of otitis. OBJECTIVE: The aim of this study was to analyse the in vitro efficacy of EOs against M. pachydermatis isolates from canine ears. MATERIALS AND METHODS: Twenty-two EOs were tested for their in vitro activity against 15 M. pachydermatis isolates by agar disc diffusion. In addition, diluted EOs were tested and volatile antifungal activity was analysed by vapour assay. RESULTS: Most EOs showed in vitro efficacy against M. pachydermatis. A different susceptibility pattern of tested isolates was found. Overall, the most potential EO was lemon grass. Also, cinnamon leaf, clove, manuka, Indian melissa, oregano, palmarosa, and winter savory oil exhibited a strong antifungal activity. Volatile activity was proven by the inhibiting effect in vapour assay. CONCLUSION:EOs show in vitro activity against M. pachydermatis. This study gives scientific background for the use of EOs in veterinary medicine. Diverse susceptibility patterns of different M. pachydermatis strains emphasise the helpfulness of an aromatogram as one lead for the best choice of an EO for therapy. The Author(s). Published by S. Karger AG, Basel.
Entities:
Keywords:
Essential oils; Malassezia pachydermatis; Otitis externa; Veterinary medicine
Authors: S Rajeshkumar; Eman F Aboelfetoh; Sri Renukadevi Balusamy; Daoud Ali; Mohammed H A Almarzoug; Jule Leta Tesfaye; Ramaswamy Krishnaraj Journal: Oxid Med Cell Longev Date: 2021-09-30 Impact factor: 6.543