Najla S Al-Harbi1, Sara T Alrashood2, Nikhat J Siddiqi1, Maha M Arafah3, Aishah Ekhzaimy4, Haseeb A Khan1. 1. Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. 2. Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. 3. Department of Pathology, College of Medicine, King Khalid University Hospital, King Saud University 12372, Riyadh, Saudi Arabia. 4. Department of Medicine, Division of Endocrinology, King Khalid University Hospital, Riyadh 12372, Saudi Arabia.
Abstract
Aim: To compare the effects of 5- and 50-nm naked and PEG-coated gold nanoparticles (AuNP) on proinflammatory cytokines (IL-1β, IL-6, TNF-α) expression and histopathological changes in liver and kidneys of rats. Materials & methods: Rats were injected with different nanoparticles and sacrificed after 24 h. Results: Both 5- and 50-nm AuNPs, and 50-nm PEG-AuNPs caused granular clumping of cytoplasm, edema and hydropic dystrophy in hepatic cells. Naked AuNPs of both sizes caused mild shrinkage, whereas 50-nm PEG-AuNPs enlarged the Bowman's space and capsule. Larger nanoparticles produced more profound mRNA expression of cytokines in both the organs. Conclusion: These findings suggest the roles of particle size and coating on immunological response and histopathological changes.
Aim: To compare the effects of 5- and 50-nm naked and PEG-coated gold nanoparticles (AuNP) on proinflammatory cytokines (IL-1β, IL-6, TNF-α) expression and histopathological changes in liver and kidneys of rats. Materials & methods: Rats were injected with different nanoparticles and sacrificed after 24 h. Results: Both 5- and 50-nm AuNPs, and 50-nm PEG-AuNPs caused granular clumping of cytoplasm, edema and hydropic dystrophy in hepatic cells. Naked AuNPs of both sizes caused mild shrinkage, whereas 50-nm PEG-AuNPs enlarged the Bowman's space and capsule. Larger nanoparticles produced more profound mRNA expression of cytokines in both the organs. Conclusion: These findings suggest the roles of particle size and coating on immunological response and histopathological changes.
Authors: Haseeb A Khan; Khalid E Ibrahim; Sara T Alrashood; Salman Alamery; Salman H Alrokayan; Najla Al-Harbi; Mohsen G Al-Mutary; Samia H Sobki; Isra Khan Journal: Saudi J Biol Sci Date: 2020-01-30 Impact factor: 4.219