| Literature DB >> 31771903 |
Florence Abravanel1, Sébastien Lhomme2, Pauline Trémeaux3, Marion Migueres3, Agnès Harter4, Catherine Haslé4, Patrick Bruel4, Laurent Alric5, Sophie Métivier5, Stéphanie Raymond2, Jacques Izopet2.
Abstract
Quantification of HBV DNA is used for initiating and monitoring antiviral treatment. We have evaluated the Xpert HBV Viral Load (VL) assay on the GeneXpert instrument. We estimated its limit of detection to be 7.5 IU/mL. Reproducibility was 1.1-12.7% as assessed by the coefficients of variation for 3 different samples. The assay was linear from 2 to 8 log10 IU/mL for HBV genotypes A to F. Its clinical performance was evaluated by testing prospectively 100 HBV DNA-positive samples with the Xpert HBV VL and Aptima Quant HBV assays. The results from the 2 assays were correlated, with a modest bias (-0.10 log10 IU/mL) between them by Bland-Altman analysis. Patient monitoring with 80 samples performed with both assays gave similar patient profiles with trends in the same direction. The Xpert HBV Viral load assay is reliable enough for quantifying HBV DNA in clinical practice.Entities:
Keywords: GeneXpert instrument; Hepatitis B virus; Molecular diagnosis
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Year: 2019 PMID： 31771903 DOI： 10.1016/j.diagmicrobio.2019.114946
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803