Cross-linked chitosan microparticles preparation by modified three fluid nozzle spray drying approach.

Owing to chitosan's incredible biocompatibility and biodegradability, bioactive molecules loaded delivery systems based on this polymer have been widely studied for improved health benefits. In the present work, quercetin and ferulic acid encapsulated core-shell structure chitosan microparticles crosslinked with tripolyphosphate and sodium alginate have been fabricated using unique three fluid nozzle (3-FN) spray drying technique. Fluorescent molecules incorporated into the microparticles aided observation of core-shell structure formation. Compatibility studies, physicochemical properties, and free radical scavenging activity of microparticles were assessed. Microscopic analysis revealed core-shell structured, spherical shaped microparticles. Thermal and non-thermal method of evaluation confirmed the crosslinking, compatibility and encapsulation of bioactive molecules with the carrier materials. The dual bioactive molecules-loaded microparticles exhibited sustained release pattern (85-88%) for 24 h and had better free radical scavenging potency. Such cross-linked core-shell structured microparticle is a promising dual delivery system for encapsulating bioactive molecules with synergistic antioxidant activity.