Perran Boran1, Hatice Ezgi Baris2, Eda Kepenekli3, Can Erzik4, Ahmet Soysal5, Duy M Dinh6. 1. Department of Pediatrics, Division of Social Pediatrics, Marmara University School of Medicine, Istanbul, Turkey. 2. Department of Pediatrics, Division of Social Pediatrics, Marmara University School of Medicine, Istanbul, Turkey. ezgi_aksu@yahoo.com. 3. Department of Pediatrics, Division of Pediatric Infectious Diseases, Marmara University School of Medicine, Istanbul, Turkey. 4. Department of Medical Biology, Marmara University School of Medicine, Istanbul, Turkey. 5. Pediatric Infectious Diseases, Memorial Hospital, Istanbul, Turkey. 6. Diversigen, Houston, USA.
Abstract
Although physiologic and neurologic consequences of micronutrient deficiencies have been addressed extensively, less is known about their impact on developing gut microbiota. Vitamin B12 deficiency is a common micronutrient deficiency in infants. We aimed to analyze the gut microbial composition of exclusively breastfed infants aged between 4 and 6 months with and without vitamin B12 deficiency by 16S rRNA gene sequencing. In a subgroup of infants with vitamin B12 deficiency, stool samples are recollected and reanalyzed after vitamin B12 supplementation. A total of 88 infants' stool samples (median age 4 months [IQR 4-5], 50% males) were analyzed, of which 28 (31.8%) were vitamin B12 sufficient and 60 (68.2%) were vitamin B12 insufficient. Comparisons between vitamin B12-sufficient and vitamin B12-insufficient infants revealed no evidence of differences in the microbiota. Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the most abundant phyla in all groups. There was no difference between the pre- and post-treatment composition of gut microbiota. Conclusion: Vitamin B12-deficient infants have similar gut microbial composition as vitamin B12-sufficient infants. Since the samples were collected at an early period of life and the exposure to deficiency was relatively short, it may be possible that the effects were not fully established.What is Known: • Vitamin B12 is an essential vitamin for humans and also a crucial compound for human gut microbiota. • Vitamin B12 deficiency is common in exclusively breastfed infants. • In contrast to the adult gut microbiota, infant gut microbiota has been shown to have decreased capacity for de novo synthesis of vitamin B12 and depend on dietary source of vitamin B12.What is New: • There is no difference in the gut microbial composition of vitamin B12-deficient and vitamin B12-sufficient infants.
Although physiologic and neurologic consequences of micronutrient deficiencies have been addressed extensively, less is known about their impact on developing gut microbiota. VitaminB12 deficiency is a common micronutrient deficiency in infants. We aimed to analyze the gut microbial composition of exclusively breastfed infants aged between 4 and 6 months with and without vitaminB12 deficiency by 16S rRNA gene sequencing. In a subgroup of infants with vitaminB12 deficiency, stool samples are recollected and reanalyzed after vitaminB12 supplementation. A total of 88 infants' stool samples (median age 4 months [IQR 4-5], 50% males) were analyzed, of which 28 (31.8%) were vitaminB12 sufficient and 60 (68.2%) were vitaminB12 insufficient. Comparisons between vitaminB12-sufficient and vitaminB12-insufficient infants revealed no evidence of differences in the microbiota. Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the most abundant phyla in all groups. There was no difference between the pre- and post-treatment composition of gut microbiota. Conclusion:VitaminB12-deficient infants have similar gut microbial composition as vitaminB12-sufficient infants. Since the samples were collected at an early period of life and the exposure to deficiency was relatively short, it may be possible that the effects were not fully established.What is Known: • VitaminB12 is an essential vitamin for humans and also a crucial compound for human gut microbiota. • VitaminB12 deficiency is common in exclusively breastfed infants. • In contrast to the adult gut microbiota, infant gut microbiota has been shown to have decreased capacity for de novo synthesis of vitaminB12 and depend on dietary source of vitaminB12.What is New: • There is no difference in the gut microbial composition of vitaminB12-deficient and vitaminB12-sufficient infants.
Entities:
Keywords:
Breastfeeding; Gut microbiota; Infants; Micronutrients; Vitamin B12
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