Injectable and thermosensitive nanofibrous hydrogel for bone tissue engineering.

The use of injectable hydrogels is currently restricted by the challenge of achieving fast gelation, good mechanical strength, and cytocompatibility. Polymeric self-assembly is a potent tool for generating functional materials that combine multiple characteristics and can react to external factors. In this study, we have developed fiber-reinforced composite hydrogels that exhibits significantly enhanced mechanical strength, reduced gelling time, and excellent cytocompatibility. The practicability of developing a chitosan-based thermogelling solution using hydroxyapatite and polyelectrolyte complex (PEC) self-assembled fibers were evaluated. The effect of βGP concentration on gelation time was studied by varying the concentration of βGP added to the chitosan solution. Various combinations were tested to create a suitable hydrogel environment for cell encapsulation, growth, and proliferation at physiological pH and temperature. Determination of Young modulus revealed that PEC fibers reinforced hydrogel was three times stiffer than chitosan-βGP gels. The gelation time was reduced to 3 min, and the hydrogels had porous structures and gels at physiological pH, temperature, and showed >80% viability for MTT assay to MG63 cells. Moreover, confocal imaging of PEC fiber reinforced hydrogels showed noticeable viability and proliferation. The molecular interactions between gelling agents, polyelectrolytes, and hydroxyapatite were studied using FTIR. We investigated interfacial bonding between PEC fibers with βGP, NaHCO3, and HAp. The combination of hydroxyapatite and polymer self-assembly technique improved the efficiency of injectable hydrogels that are helpful in minimally invasive applications.