Shujie Yan1, Brett Napiwocki2, Yiyang Xu1, Jue Zhang3, Xiang Zhang4, Xiaofeng Wang4, Wendy C Crone2, Qian Li5, Lih-Sheng Turng6. 1. School of Mechanics and Engineering Science, Zhengzhou University, Zhengzhou, China; National Center for International Research of Micro-Nano Molding Technology Zhengzhou University, Zhengzhou, China; Polymer Engineering Center, Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, WI, USA; Wisconsin Institute for Discovery University of Wisconsin-Madison, Madison, WI, USA. 2. Wisconsin Institute for Discovery University of Wisconsin-Madison, Madison, WI, USA. 3. Morgridge Institute for Research, Madison, WI, USA. 4. School of Mechanics and Engineering Science, Zhengzhou University, Zhengzhou, China; National Center for International Research of Micro-Nano Molding Technology Zhengzhou University, Zhengzhou, China. 5. School of Mechanics and Engineering Science, Zhengzhou University, Zhengzhou, China; National Center for International Research of Micro-Nano Molding Technology Zhengzhou University, Zhengzhou, China. Electronic address: qianli@zzu.edu.cn. 6. Polymer Engineering Center, Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, WI, USA; Wisconsin Institute for Discovery University of Wisconsin-Madison, Madison, WI, USA. Electronic address: turng@engr.wisc.edu.
Abstract
In this study, a small-diameter, double-layered eggshell membrane/thermoplastic polyurethane (ESM/TPU) vascular graft with a wavy structure was developed. The avian eggshell membrane, a fibrous structure similar to the extracellular matrix (ECM), has the potential to yield rapid endothelialization in vitro. The dopamine and heparin modification of the ESM surface not only promoted human umbilical vein endothelial cell (HUVEC) proliferation via cytocompatibility assessment, but also improved its anticoagulation properties as verified in platelet adhesion tests. The biomimetic mechanical properties of the vascular graft were provided by the elastic TPU fibers via electrospinning using a wavy cross-section rotating collector. The advantage of combining these two materials is to make use of the bioactivity of ESM as the internal membrane and the tunable mechanical properties of TPU as the external layer. The circumferentially wavy structure of the vascular graft produced a toe region in the non-linear section of the stress-strain curve similar to that of natural blood vessels. The ESM/TPU graft's circumferential ultimate strength was 2.57 MPa, its strain was 339% mm/mm, and its toe region was found to be around 20% mm/mm. Cyclical tension tests showed that the vascular graft could maintain good mechanical properties and showed no structural damage under repeated extension tests.
In this study, a small-diameter, double-layered eggshell membrane/thermoplastic n class="Chemical">polyurethane (ESM/TPU) vascular graft with a wavy structure was developed. The avian eggshell membrane, a fibrous structure similar to the extracellular matrix (ECM), has the potential to yield rapid endothelialization in vitro. The dopamine and heparin modification of the ESM surface not only promoted humanumbilical vein endothelial cell (HUVEC) proliferation via cytocompatibility assessment, but also improved its anticoagulation properties as verified in platelet adhesion tests. The biomimetic mechanical properties of the vascular graft were provided by the elastic TPU fibers via electrospinning using a wavy cross-section rotating collector. The advantage of combining these two materials is to make use of the bioactivity of ESM as the internal membrane and the tunable mechanical properties of TPU as the external layer. The circumferentially wavy structure of the vascular graft produced a toe region in the non-linear section of the stress-strain curve similar to that of natural blood vessels. The ESM/TPU graft's circumferential ultimate strength was 2.57 MPa, its strain was 339% mm/mm, and its toe region was found to be around 20% mm/mm. Cyclical tension tests showed that the vascular graft could maintain good mechanical properties and showed no structural damage under repeated extension tests.
Authors: Cay M Kielty; Simon Stephan; Michael J Sherratt; Matthew Williamson; C Adrian Shuttleworth Journal: Philos Trans R Soc Lond B Biol Sci Date: 2007-08-29 Impact factor: 6.237
Authors: Shahriar Mahdavi; Armin Amirsadeghi; Arman Jafari; Seyyed Vahid Niknezhad; Sidi A Bencherif Journal: Ind Eng Chem Res Date: 2021-11-23 Impact factor: 3.720
Authors: Matej Baláž; Elena V Boldyreva; Dmitry Rybin; Stefan Pavlović; Daily Rodríguez-Padrón; Tihana Mudrinić; Rafael Luque Journal: Front Bioeng Biotechnol Date: 2021-01-27
Authors: María A Rodríguez-Soto; Camilo A Polanía-Sandoval; Andrés M Aragón-Rivera; Daniel Buitrago; María Ayala-Velásquez; Alejandro Velandia-Sánchez; Gabriela Peralta Peluffo; Juan C Cruz; Carolina Muñoz Camargo; Jaime Camacho-Mackenzie; Juan Guillermo Barrera-Carvajal; Juan Carlos Briceño Journal: Polymers (Basel) Date: 2022-08-23 Impact factor: 4.967