Jianghong Zhang1,2, Meiying Xue1,2, Yue Mei1,2, Zhigang Li3, Zeng Ceng1,2, Yuanyuan Li1,2, Yi Zhang4, Na Li4, Huajing Teng5, Zhong Sheng Sun6,7,8, Yan Wang9,10. 1. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, China. 2. University of Chinese Academy of Science, Beijing, 100049, China. 3. Laboratory of Environmental Criteria and Risk Assessment & Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. 4. Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, 325000, China. 5. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, China. tenghj@biols.ac.cn. 6. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, China. sunzs@biols.ac.cn. 7. University of Chinese Academy of Science, Beijing, 100049, China. sunzs@biols.ac.cn. 8. Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, 325000, China. sunzs@biols.ac.cn. 9. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, China. wangy@biols.ac.cn. 10. University of Chinese Academy of Science, Beijing, 100049, China. wangy@biols.ac.cn.
Abstract
RATIONALE: Mood-related behavioral assays, designed typically on rodents' natural aversion to certain threats, are useful in studying the mechanisms of mood and in discovering effective treatments for neuropsychiatric disorders. OBJECTIVES: Although reasonable attention has been paid to the conducted sequence, few studies address the argument whether a behavioral assay itself affects the intrinsic signaling, gene expression, and the subsequent performance of mice. METHODS: We examined the short- (1 day) and long-term effects (7 and 14 days) of commonly used behavioral assays for anxiety and depression, including the elevated plus maze test (EPM), forced swimming test (FST), and tail suspension test (TST), on behaviors. We also investigated the effects of repeated behavioral assays on behaviors. The alterations in the expression profiles in the hippocampus experienced behavioral assays were explored via the integrative analysis of mRNA and lncRNA transcriptomes generated by RNA sequencing. RESULTS: We found that one FST or TST can induce anxiety-related behaviors, while repeated FST or TST resulted in depression-related behaviors in mice. The altered behaviors were associated with extensive transcriptional alterations in the FST and TST hippocampus of mice. KEGG pathway analyses indicated that differentially expressed genes (DEGs) in the FST and TST hippocampus were enriched in anxiety- and metabolic-related pathways, respectively. Moreover, differentially expressed lncRNAs, showing correlations with DEGs, were linked to anxiety-related pathways in the FST hippocampus and metabolic-related pathways in the TST hippocampus. CONCLUSIONS: Our study identified the unique and shared mRNAs and lncRNAs regulated by mood-related behavioral assays, emphasizing the importance of the sequence of and intervals between them.
RATIONALE: Mood-related behavioral assays, designed typically on rodents' natural aversion to certain threats, are useful in studying the mechanisms of mood and in discovering effective treatments for neuropsychiatric disorders. OBJECTIVES: Although reasonable attention has been paid to the conducted sequence, few studies address the argument whether a behavioral assay itself affects the intrinsic signaling, gene expression, and the subsequent performance of mice. METHODS: We examined the short- (1 day) and long-term effects (7 and 14 days) of commonly used behavioral assays for anxiety and depression, including the elevated plus maze test (EPM), forced swimming test (FST), and tail suspension test (TST), on behaviors. We also investigated the effects of repeated behavioral assays on behaviors. The alterations in the expression profiles in the hippocampus experienced behavioral assays were explored via the integrative analysis of mRNA and lncRNA transcriptomes generated by RNA sequencing. RESULTS: We found that one FST or TST can induce anxiety-related behaviors, while repeated FST or TST resulted in depression-related behaviors in mice. The altered behaviors were associated with extensive transcriptional alterations in the FST and TST hippocampus of mice. KEGG pathway analyses indicated that differentially expressed genes (DEGs) in the FST and TST hippocampus were enriched in anxiety- and metabolic-related pathways, respectively. Moreover, differentially expressed lncRNAs, showing correlations with DEGs, were linked to anxiety-related pathways in the FST hippocampus and metabolic-related pathways in the TST hippocampus. CONCLUSIONS: Our study identified the unique and shared mRNAs and lncRNAs regulated by mood-related behavioral assays, emphasizing the importance of the sequence of and intervals between them.
Authors: B M Andrus; K Blizinsky; P T Vedell; K Dennis; P K Shukla; D J Schaffer; J Radulovic; G A Churchill; E E Redei Journal: Mol Psychiatry Date: 2010-11-16 Impact factor: 15.992
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Authors: Luis C Reis; André S Mecawi; Verónica Trujillo; Evandro Valentim-Lima; Rodrigo Mencalha; Quézia S R Carbalan; Raoni C Dos-Santos; Viviane Felintro; Carlos E N Girardi; Rodrigo Rorato; Danilo Lustrino Journal: Mol Neurobiol Date: 2020-10-20 Impact factor: 5.590
Authors: Piotr Czarny; Katarzyna Białek; Sylwia Ziółkowska; Justyna Strycharz; Gabriela Barszczewska; Tomasz Sliwinski Journal: J Pers Med Date: 2021-03-01