| Literature DB >> 31757927 |
Karnelia Paul1, Chinmay Saha1,2, Mayurakshi Nag1, Drishti Mandal3, Haraprasad Naiya1,4, Diya Sen5,6, Souvik Mitra7, Mohit Kumar8, Dipayan Bose9, Gairik Mukherjee1, Nabanita Naskar10,11, Susanta Lahiri10, Upal Das Ghosh12, Sudipta Tripathi13, Mousumi Poddar Sarkar14, Manidipa Banerjee8, Aleysia Kleinert15, Alexander J Valentine15, Sucheta Tripathy6, Senjuti Sinharoy3, Anindita Seal16.
Abstract
Nitrogen (N) limits crop yield, and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting, N2-fixing microbes. Rhodotorula mucilaginosa JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail (Typha angustifolia). JGTA-S1 could not convert nitrate or nitrite to ammonium but harbors diazotrophic (N2-fixing) endobacteria (Pseudomonas stutzeri) that allow JGTA-S1 to fix N2 and grow in a N-free environment; moreover, P. stutzeri dinitrogen reductase was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing P. stutzeri JGTA-S1 colonizes rice (Oryza sativa), significantly improving its growth, N content, and relative N-use efficiency. Endofungal P. stutzeri plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N2-fixing ability than free-living P. stutzeri and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and NODULE INCEPTION-like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and P. stutzeri only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.Entities:
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Year: 2019 PMID： 31757927 PMCID： PMC7008492 DOI： 10.1105/tpc.19.00385
Source DB: PubMed Journal: Plant Cell ISSN： 1040-4651 Impact factor: 11.277