Mariana J C Gomes1, Sâmara L S Lima1, Natália E G Alves1, Andressa Assis1, Maria E C Moreira2, Renata C L Toledo1, Carla O B Rosa1, Olívia R Teixeira3, Priscila Z Bassinello3, Elvira G De Mejía4, Hércia S D Martino5. 1. Departamento de Nutrição e Saúde da Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil. 2. Departamento de Nutrição e Saúde da Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil; Faculdade Dinâmica do Vale do Piranga -FADIP, 205 G St, Paraiso, Ponte Nova, Minas Gerais, 35430-302, Brazil. 3. Embrapa Rice and Bean, Rodovia GO-462, Km 12. Zona Rural, Santo Antônio de Goiás, Goiás, 75375000, Brazil. 4. Department of Foods Science and Human Nutrition, University of Illinois Urbana-Champaign, 228 ERML, MC-051, 1201 West Gregory Drive, Urbana, IL, 61801, USA. 5. Departamento de Nutrição e Saúde da Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, Campus Universitário, Viçosa, Minas Gerais, 36570-000, Brazil. Electronic address: hercia@ufv.br.
Abstract
BACKGROUND AND AIMS: Common beans (Phaseolus vulgaris L.) protein hydrolysate is a source of bioactive peptides with known health benefits. The aim of this study was to evaluate the effect of common bean protein hydrolysate on lipid metabolism and endothelial function in male adult BALB/c mice fed an atherogenic diet for nine weeks. METHODS AND RESULTS: Male adult mice were divided into three experimental groups (n = 12) and fed with normal control diet; atherogenic diet and atherogenic diet added with bean protein hydrolysate (700 mg/kg/day) for nine weeks. Food intake, weight gain, lipid profile, Atherogenic Index of Plasma, inflammation biomarkers and endothelial function were evaluated. APH group presented reduced feed intake, weight gain, lipid profile, tumor necrosis factor-α, angiotensin II (94% and 79%, respectively) and increased endothelial nitric oxide synthase (62%). CONCLUSIONS: Protein hydrolysate showed hypocholesterolemic activity preventing inflammation and dysfunction of vascular endothelium, in addition to decreasing oxidative stress, indicating an adjuvant effect on reducing atherogenic risk.
BACKGROUND AND AIMS: Common beans (Phaseolus vulgaris L.) protein hydrolysate is a source of bioactive peptides with known health benefits. The aim of this study was to evaluate the effect of common bean protein hydrolysate on lipid metabolism and endothelial function in male adult BALB/c mice fed an atherogenic diet for nine weeks. METHODS AND RESULTS: Male adult mice were divided into three experimental groups (n = 12) and fed with normal control diet; atherogenic diet and atherogenic diet added with bean protein hydrolysate (700 mg/kg/day) for nine weeks. Food intake, weight gain, lipid profile, Atherogenic Index of Plasma, inflammation biomarkers and endothelial function were evaluated. APH group presented reduced feed intake, weight gain, lipid profile, tumor necrosis factor-α, angiotensin II (94% and 79%, respectively) and increased endothelial nitric oxide synthase (62%). CONCLUSIONS: Protein hydrolysate showed hypocholesterolemic activity preventing inflammation and dysfunction of vascular endothelium, in addition to decreasing oxidative stress, indicating an adjuvant effect on reducing atherogenic risk.