Aerobic cometabolism of 1,4-dioxane by isobutane-utilizing microorganisms including Rhodococcus rhodochrous strain 21198 in aquifer microcosms: Experimental and modeling study.

Aerobic cometabolism of the emerging contaminant 1,4-dioxane (1,4-D) by isobutane-utilizing microorganisms was assessed in pure culture and aquifer microcosm studies. The bacterium Rhodococcus rhodochrous strain ATCC 21198 transformed low, environmentally-relevant concentrations of 1,4-D when grown on isobutane. Microcosms were constructed with aquifer solids from Fort Carson, Colorado, a site contaminated with 1,4-D and trichloroethene (TCE). Multiple additions of isobutane and 1,4-D over 300 days were transformed in microcosms biostimulated with isobutane and microcosms bioaugmented with strain 21198. Results showed that, over time and with sufficient inorganic nutrients, biostimulation of native microorganisms with isobutane was just as effective as bioaugmentation with strain 21198 to achieve 1,4-D transformation in the microcosms. The presence of TCE at 0.2 mg/L did not inhibit 1,4-D transformation, though TCE itself was not readily transformed. An iterative process was used to determine kinetic parameter values to fit Michaelis-Menten/Monod models to experimental data for simultaneous isobutane utilization, biomass growth, and cometabolic transformation of 1,4-D. Parameter optimization resulted in good model fit to the data over multiple transformations of isobutane and 1,4-D in both short- and long-term experiments. Results suggest low concentrations of 1,4-D studied in the microcosms were cometabolically transformed according to a pseudo first-order rate of 0.37 L/mg TSS/day of 21198. Isobutane consumption was modeled with a maximum rate of 2.58 mg/mg TSS/day and a half saturation constant of 0.09 mg/L. 1,4-D transformation was competitively inhibited by the presence of isobutane and transformation rates were significantly reduced when inorganic nutrients were limiting. Simulations of the repeated additions found a first-order microbial endogenous decay coefficient of 0.03 day-1 fit the alternating periods of active transformation and stagnation between isobutane and 1,4-D additions over approximately one year. The model fitting process highlighted the importance of determining kinetic parameters from data representing low concentrations typically found in the environment.