Xing Zhang1, Zi Liang1, Yunshan Zhang1, Kun Dai1, Min Zhu1, Ji Wang2, Xiaolong Hu3. 1. School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China. 2. Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China. Electronic address: royking_333@163.com. 3. School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China. Electronic address: xlhu2013@suda.edu.cn.
Abstract
BACKGROUND: Long non-coding RNAs (lncRNAs) play crucial roles in the progression and pathogenesis of cancer. Right now, less is known about the association between the expression of lncRNAs and the pathogenesis of pulmonary tuberculosis (PTB). METHODS: In present study, the expression profiles of lncRNAs were investigated by transcriptome sequencing from PTB patients vs. healthy individuals. RESULTS: A total of 449 differentially expressed (DE) (fold change ≥2, false discovery rate ≤ 0.05) lncRNAs were screened out from the PTB patients. Lnc-HNRNPU-1:7 and lnc-FAM76B-4:1 was found the most upregulated lncRNAs and downregulated lncRNAs in PTB patients, respectively. GO annotation and KEGG analysis were used to explore the potential roles of these DE lncRNAs. The JAK/STAT and TGF-β signaling pathways related to PTB pathogenesis were enriched in PTB patients. The co-expressed of a few lncRNAs and mRNAs on chromosome were shown by cis-regulatory gene analysis. Trans analysis indicated that STAT1, STAT2 and TAF7 transcription factors regulated the expression of lncRNA and mRNA. The constructed lncRNA ceRNA network suggested that lncRNAs regulating mRNAs expression may mediate by sponged miRNAs. CONCLUSION: We comprehensively analyzed the expression profiles of lncRNAs in PTB patients, thus providing new clues for exploring the regulatory mechanisms of dysregulated lncRNAs in the pathogenesis of PTB.
BACKGROUND: Long non-coding RNAs (lncRNAs) play crucial roles in the progression and pathogenesis of cancer. Right now, less is known about the association between the expression of lncRNAs and the pathogenesis of pulmonary tuberculosis (PTB). METHODS: In present study, the expression profiles of lncRNAs were investigated by transcriptome sequencing from PTBpatients vs. healthy individuals. RESULTS: A total of 449 differentially expressed (DE) (fold change ≥2, false discovery rate ≤ 0.05) lncRNAs were screened out from the PTBpatients. Lnc-HNRNPU-1:7 and lnc-FAM76B-4:1 was found the most upregulated lncRNAs and downregulated lncRNAs in PTBpatients, respectively. GO annotation and KEGG analysis were used to explore the potential roles of these DE lncRNAs. The JAK/STAT and TGF-β signaling pathways related to PTB pathogenesis were enriched in PTBpatients. The co-expressed of a few lncRNAs and mRNAs on chromosome were shown by cis-regulatory gene analysis. Trans analysis indicated that STAT1, STAT2 and TAF7 transcription factors regulated the expression of lncRNA and mRNA. The constructed lncRNA ceRNA network suggested that lncRNAs regulating mRNAs expression may mediate by sponged miRNAs. CONCLUSION: We comprehensively analyzed the expression profiles of lncRNAs in PTBpatients, thus providing new clues for exploring the regulatory mechanisms of dysregulated lncRNAs in the pathogenesis of PTB.
Authors: Hong Yan; Guoye Liu; Yuan Liang; Wei Wu; Rui Xia; Lin Jiao; Han Shen; Zhijun Jia; Qian Wang; Zhiqiang Wang; Yi Kong; Binwu Ying; Hualiang Wang; Chengbin Wang Journal: Ann Transl Med Date: 2021-06