Draft Genome Sequences of Type Strains of Adlercreutzia muris and Ellagibacter urolithinifaciens, Belonging to the Family Eggerthellaceae.

Here, we report the annotated draft genome sequences of two type strains belonging to the family Eggerthellaceae within the class Coriobacteriia (phylum Actinobacteria), Adlercreutzia muris WCA-131-CoC-2 (= DSM 29508 = KCTC 15543) and Ellagibacter urolithinifaciens CEBAS 4A (= CCUG 70284 = DSM 104140).

ANNOUNCEMENT

Species of the family Eggerthellaceae are typical members of the mammalian gut and have been isolated from, e.g., humans (1, 2), mice (3–5), and sheep (6). Several Eggerthellaceae species have been reported to metabolize secondary plant compounds, especially polyphenols like ellagic acid (2), daidzein (3), and resveratrol (7). Ellagibacter urolithinifaciens CEBAS 4AT was isolated from the feces of a 41-year-old healthy male during the search for a bacterial strain that is able to metabolize ellagic acid. This strain is capable of metabolizing ellagic acid into isourolithin A (2). The type strain of Adlercreutzia muris was isolated as Enterorhabdus muris from the colonic content of a wild-type C57BL/6 mouse (5, 8). The genus Enterorhabdus showed only a low genetic divergence with the genus Adlercreutzia and has to be seen as a later heterotypic synonym of the previously described genus Adlercreutzia (9, 10).

In this study, we announce the annotated draft genome sequences of the type strains of Adlercreutzia muris and Ellagibacter urolithinifaciens. With this announcement, we provide the remaining annotated draft genome sequences of type strains of the family Eggerthellaceae with standing names in nomenclature (11).

The following type strains were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ): A. muris DSM 29508 and E. urolithinifaciens DSM 104140. Strains were cultured, and genomic DNA was isolated as previously described (1, 12, 13). Adlercreutzia muris DSM 29508T was cultured (37°C) under anaerobic conditions of N2-CO2 (80:20) in flushed brain heart infusion medium (Merck) supplemented with 0.5% yeast extract, 0.05% l-cysteine monohydrochloride (Roth), 1 mg ml−1 resazurin sodium salt, 2.5 mg liter−1 heme solution, and 2 μg ml−1 vitamin K1 solution (Sigma-Aldrich). Ellagibacter urolithinifaciens DSM 104140T was cultured (37°C) under anaerobic conditions of N2-CO2 (80:20) in flushed anaerobe basal broth (Oxoid). DNA extraction was done using a Qiagen blood and tissue kit. DNA was quantified with a double-stranded DNA (dsDNA) high-sensitivity (HS) assay on a Qubit version 2.0 fluorometer (Thermo Fisher Scientific), according to the manufacturer’s instructions, and adjusted to a concentration of 0.2 ng μl−1.

The sequencing library was constructed using a Nextera XT DNA library prep kit and a Nextera XT index kit (Illumina). Whole-genome shotgun sequencing was performed on an Illumina MiSeq benchtop sequencer using a 500-cycle v2 kit (read length, 2 × 250 bp). In total, 1,469,853 reads were generated for A. muris DSM 29508T, while 1,067,866 reads were generated for E. urolithinifaciens DSM 104140T. Data processing was done as previously described (12, 13). Sequence reads were quality trimmed using Trimmomatic version 0.39 (14) and assembled using SPAdes version 3.13.1 with default settings in the “careful” mode (15, 16). The estimated insert sizes of A. muris DSM 29508T and E. urolithinifaciens DSM 104140T were 255.724 bp and 297.597 bp, respectively. Adequate trimming was verified by mapping the adapter sequences to the assembled contigs using Bowtie 2 version 2.3.3.1 (17). To eliminate sequence contamination, the contigs were aligned to the genome of coliphage phi-X174 (GenBank accession number NC_001422) using a BLASTn search (18). All contigs of <500 bp were manually excluded, and renaming of contigs was done using awk (19). To calculate genome coverage of each strain, trimmed reads were mapped against remaining contigs by Bowtie 2. In the case of A. muris DSM 29508T, 99.15% of 1,463,425 trimmed reads could be mapped against the respective contigs, whereas in the case of E. urolithinifaciens DSM 104140T, 99.18% of 1,064,956 trimmed reads could be mapped against the contigs. Draft genome sequences were annotated using the automated NCBI Prokaryotic Genome Annotation Pipeline (20). The assembly metrics and annotated features of both type strains are given in Table 1. ResFinder version 3.2 (21) was used to evaluate the draft genome sequences for antimicrobial resistance (AMR) genes. For both strains, no AMR gene was identified.

TABLE 1

Accession numbers, assembly metrics, and annotated features of the type strains of Adlercreutzia muris and Ellagibacter urolithinifaciens

Bacterial species (strain)	Genome size (bp)	No. of contigs (avg coverage [×])	N50 (bp)	Total no. of genes	G+C content (%)	GenBank accession no.
Adlercreutzia muris (DSM 29508)	2,765,182	94 (134.19)	79,375	2,303	65.1	WAJS00000000
Ellagibacter urolithinifaciens (DSM 104140)	2,462,117	81 (127.67)	100,872	2,161	60.4	WAJR00000000

Data availability.

This whole-genome shotgun project, including raw reads of A. muris DSM 29508T and E. urolithinifaciens DSM 104140T, has been deposited at DDBJ/ENA/GenBank under BioProject number PRJNA574580. The versions described in this publication are the first versions and are listed in Table 1. The raw reads of A. muris DSM 29508T have been deposited under SRA accession number SRX6974936, while the raw reads of E. urolithinifaciens DSM 104140T have been deposited under accession number SRX6974937.