Yu Jeong Kim1, Yeji Yeon1, Won June Lee1, Yong Un Shin1, Heeyoon Cho1, Yoon-Kyoung Sung2, Du Roo Kim1, Han Woong Lim1, Min Ho Kang1,3. 1. Department of Ophthalmology, Hanyang University Hospital, Hanyang University College of Medicine, Seoul, Republic of Korea. 2. Department of Rheumatology, Hanyang University Hospital for Rheumatic Diseases, Seoul, Republic of Korea. 3. Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland, United States.
Abstract
Purpose: Deregulated expression of several microRNAs (miRNAs) in sera or salivary glands of patients with Sjögren syndrome (SS) has been reported. However, none have investigated miRNAs in samples that can represent lacrimal glands. We compared the miRNAs expression in the tears of SS patients and healthy controls. Moreover, we investigated the correlation between miRNAs expression and ocular staining score (OSS). Methods: Individual tear samples were collected from 18 SS patients and 8 age-matched controls. Clinical ophthalmologic assessments included Schirmer I test, tear film breakup time (tBUT), and OSS. The expression of 43 different miRNAs in tears was measured using real-time polymerase chain reaction, and compared between the SS patients and controls. And we also compared between the three groups of control, primary SS, and secondary SS patients. The correlation between the miRNA expression and OSS was evaluated. Results: The expression levels of miR-16-5p, miR-34a-5p, miR-142-3p, and miR-223-3p were significantly upregulated in patients with SS when compared with those in the control group (P < 0.05). The expression of 10 miRNAs (miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-92a-3p, miR-134-5p, miR-137, miR-302d-5p, miR-365b-3p, miR-374c-5p, miR-487b-3p) was significantly downregulated in the SS patients (P < 0.05). Eight miRNAs showed statistically significant differences between the three groups of control, primary SS and secondary SS. All 14 miRNAs with significant differences in SS patients and control group were not significantly correlated with OSSs. Conclusions: The 14 differentially expressed miRNAs may be involved in the pathogenesis of SS, in particular, related to autoimmunity and neuropathy.
Purpose: Deregulated expression of several microRNAs (miRNAs) in sera or salivary glands of patients with Sjögren syndrome (SS) has been reported. However, none have investigated miRNAs in samples that can represent lacrimal glands. We compared the miRNAs expression in the tears of SSpatients and healthy controls. Moreover, we investigated the correlation between miRNAs expression and ocular staining score (OSS). Methods: Individual tear samples were collected from 18 SSpatients and 8 age-matched controls. Clinical ophthalmologic assessments included Schirmer I test, tear film breakup time (tBUT), and OSS. The expression of 43 different miRNAs in tears was measured using real-time polymerase chain reaction, and compared between the SSpatients and controls. And we also compared between the three groups of control, primary SS, and secondary SSpatients. The correlation between the miRNA expression and OSS was evaluated. Results: The expression levels of miR-16-5p, miR-34a-5p, miR-142-3p, and miR-223-3p were significantly upregulated in patients with SS when compared with those in the control group (P < 0.05). The expression of 10 miRNAs (miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-92a-3p, miR-134-5p, miR-137, miR-302d-5p, miR-365b-3p, miR-374c-5p, miR-487b-3p) was significantly downregulated in the SSpatients (P < 0.05). Eight miRNAs showed statistically significant differences between the three groups of control, primary SS and secondary SS. All 14 miRNAs with significant differences in SSpatients and control group were not significantly correlated with OSSs. Conclusions: The 14 differentially expressed miRNAs may be involved in the pathogenesis of SS, in particular, related to autoimmunity and neuropathy.
Authors: Bo Liu; Lu Zhao; Yankai Wei; Sisi Chen; Lingzhai Bian; Di Guo; Min Gao; Hong Nian Journal: Int J Med Sci Date: 2020-10-16 Impact factor: 3.738
Authors: Shruti Singh Kakan; Maria C Edman; Alexander Yao; Curtis T Okamoto; Annie Nguyen; Brooke E Hjelm; Sarah F Hamm-Alvarez Journal: Front Immunol Date: 2022-03-04 Impact factor: 7.561