Sulfidogenic anaerobic digestion of sulfate-laden waste activated sludge: Evaluation on reactor performance and dynamics of microbial community.

This study investigated the impact of sludge retention times (SRTs: 40, 20, 10 and 5 days) on performance of the sulfidogenic anaerobic digestion (SAD) reactor treating sulfate-laden waste activated sludge and dynamics of sulfate reducing bacteria (SRB). The findings showed that sulfide production, volatile sludge removal efficiency, ammonia release and methane yield decreased by 33.7%, 66.4%, 21.3% and 68.7%, respectively when SRT was shortened from 40 to 5 d. Significant enrichment of hydrolyzers/fermenters (genera Mesotoga and Sulfurovum) was observed at longer SRT (40 d), but shorter SRT (5 d) favors enrichment of diverse SRB (genera Desulfomicrobium and Desulfovibrio). PICRUSt data revealed bacterial communities possessed diverse predicted functions including sulfur metabolism enzymes (e.g. sulfate adenylyltransferase), and their abundance was higher at shorter SRT. Statistical analysis (PCA) confirmed positive relationships between SRB and SAD performance. The findings of this research could be useful for design and optimization of sulfidogenic-based anaerobic digestion process.