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Literature DB >> 31746923

Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR.

Zhenhao Zhang¹, Xiao Shan, Pengbo Zhang, Weiliang Liu, Jingli Yan, Zhengping Li.

Abstract

A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.

Entities: Chemical

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Year: 2019 PMID： 31746923 DOI： 10.1039/c9ob02429h

Source DB: PubMed Journal: Org Biomol Chem ISSN： 1477-0520 Impact factor: 3.876

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Cited

1 in total

1. Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP.

Authors: Zhenhao Zhang; Tong He; Yan Qi; Yuxuan Dai; Kejing Lao; Xingchun Gou
Journal: RSC Adv Date: 2022-07-07 Impact factor: 4.036

1 in total