Xiao Hu 1 , Min Wang 2 , Lei Cao 1 , Li Cong 1 , Yujie Gao 1 , Jianwei Lu 3 , Jifeng Feng 3 , Bo Shen 3 , Delin Liu 3 Show Affiliations »
Abstract
BACKGROUND: The functions of microRNAs (miRNAs) in cancer progression have been recognized in recent years. However, the role of miR-4319 in esophageal squamous cell carcinoma (ESCC) remains unclear. OBJECTIVE: We aimed to investigate the biological roles of miR-4319 in ESCC progression and the associated mechanisms. METHODS: Real-time PCR was performed to examine the levels of miR-4319 in ESCC cell lines. The effects of miR-4319 and NOD-like receptor (NLR) family, caspase activation and recruitment domain (CARD) domain containing 5 (NLRC5) on cell proliferation and cell cycle progression were evaluated using MTT assay, colony formation and flow cytometry assays. Bioinformatics techniques and luciferase reporter assay were applied to validate NLRC5 as a miR-4319 target. RESULTS: The miR-4319 expression was lower in ESCC cells than in the normal cell line. The expression of miR-4319 repressed cell growth and induced cell cycle arrest. NLRC5 was validated as a direct downstream target of miR-4319. Overexpression of NLRC5 potentiated the effects of miR-4319 on cell growth and cell cycle distribution. CONCLUSION: Our results demonstrated that miR-4319 might function as a tumor suppressor by targeting NLRC5 in ESCC. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
BACKGROUND: The functions of microRNAs (miRNAs) in cancer progression have been recognized in recent years. However, the role of miR-4319 in esophageal squamous cell carcinoma (ESCC ) remains unclear. OBJECTIVE: We aimed to investigate the biological roles of miR-4319 in ESCC progression and the associated mechanisms. METHODS: Real-time PCR was performed to examine the levels of miR-4319 in ESCC cell lines. The effects of miR-4319 and NOD-like receptor (NLR) family, caspase activation and recruitment domain (CARD) domain containing 5 (NLRC5 ) on cell proliferation and cell cycle progression were evaluated using MTT assay, colony formation and flow cytometry assays. Bioinformatics techniques and luciferase reporter assay were applied to validate NLRC5 as a miR-4319 target. RESULTS: The miR-4319 expression was lower in ESCC cells than in the normal cell line. The expression of miR-4319 repressed cell growth and induced cell cycle arrest . NLRC5 was validated as a direct downstream target of miR-4319 . Overexpression of NLRC5 potentiated the effects of miR-4319 on cell growth and cell cycle distribution. CONCLUSION: Our results demonstrated that miR-4319 might function as a tumor suppressor by targeting NLRC5 in ESCC . Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities: Chemical
Disease
Gene
Keywords:
NLRC5; cell culture; cell cycle; cell growth; esophageal squamous cell carcinoma; miR-4319.
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Year: 2020
PMID： 31746301 DOI： 10.2174/1874467212666191119094636
Source DB: PubMed Journal: Curr Mol Pharmacol ISSN： 1874-4672 Impact factor: 3.339