Cardiorespiratory reflex responses to static contraction of vascularly isolated hindleg muscles of the rat.

One hindleg of an anasthetized rat (n = 15) was isolated from systemic blood circulation. The preparation was connected to the body only by nerve and bone. A. and V. femorales were cannulated and perfused with normoxic (PO2 = 530 mm Hg) or hypoxic (PO2 = 60 mm Hg) Tyrode solutions. Static contractions of the muscle were elicited by electrical stimulation on the sciatic nerve (2 x motor threshold, 400-800 mV, 50 s-1). A 1 s stimulus was followed by a 2 s rest period. Total test time amounted to 40 min. It was proceeded and succeeded by 20 min periods of control perfusions without stimulation. Heart rate (HR) and respiratory rate (f) were measured and cross correlated with the following outflow parameters from V. femoralis of the experimental muscle: [K+], [Na+], PO2, PCO2, pH and [lactate]. During the test period HR and f increased significantly within 20 min of the start of stimulation: HR 5.8% (p less than 0.005) and f 24.3% (p less than 0.005) for hypoxic perfusion (n = 6) and HR 3.2% (p less than 0.005) and f (p less than 0.001, ANOVA) for normoxic perfusion (n = 3). The dynamic changes of several outflow parameters were nearly simultaneous with the cardiorespiratory responses. Cross correlation analyses revealed an excellent temporal relationship between HR and PO2 or [lactate] and between f and PO2 or [lactate]. In addition PCO2 and pH correlated well with HR as well as with f. Comparison of the threshold of the cardiorespiratory response revealed an optimal relationship to pH, a good one to PCO2 and lactate concentration but no correlation to PO2.(ABSTRACT TRUNCATED AT 250 WORDS)