Salvador García-López1, Rosina E Villanueva2, Felipe Massó-Rojas3, Araceli Páez-Arenas4, Murray C Meikle5. 1. Health Science Department/Cell Biology and Immunology Laboratory, Universidad Autónoma Metropolitana-Xochimilco, 04960, Mexico City, Mexico; Orthodontic Department, General Hospital "Dr. Manuel Gea González", UNAM, 14080, Mexico City, Mexico; Universidad Intercontinental, Mexico City, Mexico. Electronic address: sgarcia@correo.xoc.uam.mx. 2. School of Dentistry, Universidad Autónoma Metropolitana, 04960, Mexico City, Mexico. Electronic address: rvilla@correo.xoc.uam.mx. 3. Translational Medicine Unit, Instituto Nacional de Cardiología "Ignacio Chávez", 14080, Mexico City, Mexico. Electronic address: f_masso@yahoo.com. 4. Translational Medicine Unit, Instituto Nacional de Cardiología "Ignacio Chávez", 14080, Mexico City, Mexico. 5. Emeritus Professor King's College, Dental Institute, at Guy's, King's and St. Thomas's Hospital, University of London, SE1 9RT, United Kingdom. Electronic address: murray.meikle@cantab.net.
Abstract
OBJETIVE: It has been claimed that micro-pulse vibration can accelerate the rate of tooth movement during orthodontic treatment; however, the underlying cellular mechanism has yet to be elucidated. The purpose of this study was to understand the mechanisms underlying tooth movement acceleration by measuring alterations in a panel of intercellular signalling molecules and markers of osteoblast/osteoclast function following micro-pulse vibration for 20 min at 30 Hz. DESIGN: Primary BALB/c mouse calvarial osteoblasts were cultivatedin vitro and subjected to micro-pulse vibration (0.25 N; 30 Hz) with the AcceleDent® Aura appliance for 20 min and assayed for IL-4, IL-13, IL-17, OPG, soluble RANKL and TGF-β protein by ELISA; for PCNA in osteoblasts and caspase 3/7 in osteoclasts by immunohistochemistry; for IL-4, IL-13, and Il-17 in osteoclasts by ELISA; and for cathepsin K by flow cytometry. RESULTS: After micro-pulse vibration, the murine osteoblast culture supernatant showed increased IL-4, IL-13, IL-17, OPG and TGF-β levels and decreased RANKL levels; PCNA in osteoblasts and caspase 3/7 in osteoclasts were also upregulated. The osteoclast culture supernatant had increased levels of IL-4, IL-13 and IL-17, and cathepsin K was upregulated in the treatment group compared with the control group. CONCLUSIONS: Micro-pulse vibration promotes the production of soluble factors that inhibit osteoclasts, promote apoptosis and activate osteoblasts in vitro, which could increase bone mineral density. Further studies should be conducted in order to understand the biological mechanism of how micro-vibration might influence tooth movement during orthodontic treatment.
OBJETIVE: It has been claimed that micro-pulse vibration can accelerate the rate of tooth movement during orthodontic treatment; however, the underlying cellular mechanism has yet to be elucidated. The purpose of this study was to understand the mechanisms underlying tooth movement acceleration by measuring alterations in a panel of intercellular signalling molecules and markers of osteoblast/osteoclast function following micro-pulse vibration for 20 min at 30 Hz. DESIGN: Primary BALB/c mouse calvarial osteoblasts were cultivatedin vitro and subjected to micro-pulse vibration (0.25 N; 30 Hz) with the AcceleDent® Aura appliance for 20 min and assayed for IL-4, IL-13, IL-17, OPG, soluble RANKL and TGF-β protein by ELISA; for PCNA in osteoblasts and caspase 3/7 in osteoclasts by immunohistochemistry; for IL-4, IL-13, and Il-17 in osteoclasts by ELISA; and for cathepsin K by flow cytometry. RESULTS: After micro-pulse vibration, the murine osteoblast culture supernatant showed increased IL-4, IL-13, IL-17, OPG and TGF-β levels and decreased RANKL levels; PCNA in osteoblasts and caspase 3/7 in osteoclasts were also upregulated. The osteoclast culture supernatant had increased levels of IL-4, IL-13 and IL-17, and cathepsin K was upregulated in the treatment group compared with the control group. CONCLUSIONS: Micro-pulse vibration promotes the production of soluble factors that inhibit osteoclasts, promote apoptosis and activate osteoblasts in vitro, which could increase bone mineral density. Further studies should be conducted in order to understand the biological mechanism of how micro-vibration might influence tooth movement during orthodontic treatment.
Authors: Mehmet Ali Karabel; Mehmet Doğru; Arzum Doğru; Mehmet İrfan Karadede; Mehmet Cudi Tuncer Journal: Acta Cir Bras Date: 2021-01-20 Impact factor: 1.388