Konstantin Rozenberg1, Ayala Wollman2, Michaella Ben-Shachar3, Lital Argaev-Frenkel4, Tovit Rosenzweig5. 1. Department of Molecular Biology, Ariel University, Ariel, Israel; Department of Nutrition Sciences, Ariel University, Ariel, Israel. Electronic address: Rozenberg.kos@gmail.com. 2. Department of Molecular Biology, Ariel University, Ariel, Israel; Department of Nutrition Sciences, Ariel University, Ariel, Israel. Electronic address: Ayalalumer22@gmail.com. 3. Department of Molecular Biology, Ariel University, Ariel, Israel; Department of Nutrition Sciences, Ariel University, Ariel, Israel. Electronic address: 0584409512bs@gmail.com. 4. Department of Molecular Biology, Ariel University, Ariel, Israel; Department of Nutrition Sciences, Ariel University, Ariel, Israel. Electronic address: litalfren@gmail.com. 5. Department of Molecular Biology, Ariel University, Ariel, Israel; Department of Nutrition Sciences, Ariel University, Ariel, Israel. Electronic address: tovitro@ariel.ac.il.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Sarcopoterium spinosum is a Mediterranean plant, widely used by the Beduin traditional medicine. While its major use is for the treatment of diabetes, there are also documentations for its use as anti-inflammatory agent. This therapeutic potential of Sarcopoterium spinosum was not validated before. AIM OF THE STUDY: To investigate the anti-inflammatory properties of Sarcopoterium spinosum extract (SSE). MATERIALS AND METHODS: Experiments were performed on RAW264.7 macrophages and bone marrow-derived macrophages (BMDM) and the effect of SSE on markers of inflammation was investigated. In addition, the effect of SSE on the development of inflammation in adipose-tissue of obese, insulin resistant mice was measured in KK-Ay mice and high fat diet (HFD)-fed mice. RESULTS: Lipopolysaccharide (LPS) and SSE increased the viability of RAW264.7. In addition, the cells acquired distinct dendritic-like morphology, however, while LPS induced NO production and the mRNA expression of pro-inflammatory cytokines, SSE increased the mRNA expression of anti-inflammatory genes and blocked LPS effects. All three pathways of MAPK were activated by LPS and SSE, as demonstrated by the phosphorylation of ERK, p38 and JNK. NFκB was activated and Akt was phosphorylated by LPS, while SSE blocked this effects. STAT proteins were differently phosphorylated by SSE and LPS. Immunomodulatory effects of SSE were also found in BMDM. In adipose tissue of SSE-treated mice, less crown-like structures were found, and lower expression of pro-inflammatory adipocytokines was observed, although adipocytes hypertrophy was not affected. CONCLUSIONS: SSE has an immunomodulatory effects that affect macrophage function, and reduces adipose tissue inflammation. Identifying active component and clarifying its mechanism of action might support the development of new anti-inflammatory agent.
ETHNOPHARMACOLOGICAL RELEVANCE: Sarcopoterium spinosum is a Mediterranean plant, widely used by the Beduin traditional medicine. While its major use is for the treatment of diabetes, there are also documentations for its use as anti-inflammatory agent. This therapeutic potential of Sarcopoterium spinosum was not validated before. AIM OF THE STUDY: To investigate the anti-inflammatory properties of Sarcopoterium spinosum extract (SSE). MATERIALS AND METHODS: Experiments were performed on RAW264.7 macrophages and bone marrow-derived macrophages (BMDM) and the effect of SSE on markers of inflammation was investigated. In addition, the effect of SSE on the development of inflammation in adipose-tissue of obese, insulin resistant mice was measured in KK-Ay mice and high fat diet (HFD)-fed mice. RESULTS: Lipopolysaccharide (LPS) and SSE increased the viability of RAW264.7. In addition, the cells acquired distinct dendritic-like morphology, however, while LPS induced NO production and the mRNA expression of pro-inflammatory cytokines, SSE increased the mRNA expression of anti-inflammatory genes and blocked LPS effects. All three pathways of MAPK were activated by LPS and SSE, as demonstrated by the phosphorylation of ERK, p38 and JNK. NFκB was activated and Akt was phosphorylated by LPS, while SSE blocked this effects. STAT proteins were differently phosphorylated by SSE and LPS. Immunomodulatory effects of SSE were also found in BMDM. In adipose tissue of SSE-treated mice, less crown-like structures were found, and lower expression of pro-inflammatory adipocytokines was observed, although adipocytes hypertrophy was not affected. CONCLUSIONS: SSE has an immunomodulatory effects that affect macrophage function, and reduces adipose tissue inflammation. Identifying active component and clarifying its mechanism of action might support the development of new anti-inflammatory agent.
Authors: Valid Gahramanov; Moria Oz; Tzemach Aouizerat; Tovit Rosenzweig; Jonathan Gorelick; Elyashiv Drori; Mali Salmon-Divon; Michael Y Sherman; Bat Chen R Lubin Journal: Plants (Basel) Date: 2022-08-24