Ziyan Zhu1, Weiwei Cui1, Dimin Zhu2, Nailin Gao2, Yonghong Zhu3. 1. Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China. 2. Department of Neurosurgery and Pituitary Tumor Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China. 3. Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China. zhuyongh@mail.sysu.edu.cn.
Abstract
PURPOSE: Pituitary tumor is the common primary brain tumor in humans. For further studying the pathogenesis and new therapeutic targets of pituitary adenoma, cell lines and primary cells are necessary tools. Different from primary cells that have short survival time and hormone secretion maintenance time, cell lines would be endowed with immortal characteristics under the help of gene modification. This review is to explore whether these cell lines still have similar pathophysiological changes in pituitary adenoma cells and methods to prolong the lifespan of pituitary adenoma primary cells. RESULTS: In the cell lines summarized in the review, HP75, PDFS, HPA and GX were derived from human pituitary adenomas. It was found that the cell lines commonly used in articles published between January 2014 and July 2019 were GH3, AtT20, MMQ, GH4C1, HP75 and TtT/GF. Besides, it was glad that many methods had been used to prolong the lifespan and maintain characteristics of pituitary adenoma primary cells. CONCLUSION: The paper reviews most of pituitary adenoma cell lines that have been successfully established since 1968 and the relevant situation of primary culture of pituitary adenoma cells. Obviously, it requires us to make more efforts to obtain human pituitary adenoma cell lines and prolong the lifespan of pituitary adenoma primary cells with maintaining their morphology and ability to secret hormones.
PURPOSE:Pituitary tumor is the common primary brain tumor in humans. For further studying the pathogenesis and new therapeutic targets of pituitary adenoma, cell lines and primary cells are necessary tools. Different from primary cells that have short survival time and hormone secretion maintenance time, cell lines would be endowed with immortal characteristics under the help of gene modification. This review is to explore whether these cell lines still have similar pathophysiological changes in pituitary adenoma cells and methods to prolong the lifespan of pituitary adenoma primary cells. RESULTS: In the cell lines summarized in the review, HP75, PDFS, HPA and GX were derived from humanpituitary adenomas. It was found that the cell lines commonly used in articles published between January 2014 and July 2019 were GH3, AtT20, MMQ, GH4C1, HP75 and TtT/GF. Besides, it was glad that many methods had been used to prolong the lifespan and maintain characteristics of pituitary adenoma primary cells. CONCLUSION: The paper reviews most of pituitary adenoma cell lines that have been successfully established since 1968 and the relevant situation of primary culture of pituitary adenoma cells. Obviously, it requires us to make more efforts to obtain humanpituitary adenoma cell lines and prolong the lifespan of pituitary adenoma primary cells with maintaining their morphology and ability to secret hormones.
Authors: D C Danila; X Zhang; Y Zhou; G R Dickersin; J A Fletcher; E T Hedley-Whyte; M K Selig; S R Johnson; A Klibanski Journal: J Clin Endocrinol Metab Date: 2000-03 Impact factor: 5.958
Authors: Martin Franke; Adrian F Daly; Leonor Palmeira; Amit Tirosh; Antonio Stigliano; Eszter Trifan; Fabio R Faucz; Dayana Abboud; Patrick Petrossians; Juan J Tena; Eleonora Vitali; Andrea G Lania; José L Gómez-Skarmeta; Albert Beckers; Constantine A Stratakis; Giampaolo Trivellin Journal: Am J Hum Genet Date: 2022-02-23 Impact factor: 11.043