Jie Luo1,2, Esra Abaci Turk2, Borjan Gagoski2, Natalie Copeland2, Iris Y Zhou3, Vanessa Young2, Carolina Bibbo4, Julian N Robinson4, Chloe Zera4, William H Barth5, Drucilla J Roberts6, Phillip Zhe Sun3,7, P Ellen Grant2. 1. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. 2. Fetal-Neonatal Neuroimaging and Developmental Science Center, Boston Children's Hospital, Boston, MA, USA. 3. Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, USA. 4. Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, MA, USA. 5. Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA, USA. 6. Department of Pathology, Massachusetts General Hospital, Boston, MA, USA. 7. Department of Radiology and Imaging Sciences, Emory University School of Medicine, Atlanta, GA, USA.
Abstract
BACKGROUND: To investigate dynamic glucose enhanced (DGE) chemical exchange saturation transfer (CEST) MRI as a means to non-invasively image glucose transport in the human placenta. METHODS: Continuous wave (CW) CEST MRI was performed at 3.0 Tesla. The glucose contrast enhancement (GCE) was calculated based on the magnetization transfer asymmetry (MTRasym), and the DGE was calculated with the positive side of Z-spectra in reference to the first time point. The glucose CEST (GlucoCEST) was optimized using a glucose solution phantom. Glucose solution perfused ex vivo placenta tissue was used to demonstrate GlucoCEST MRI effect. The vascular density of ex vivo placental tissue was evaluated with yellow dye after MRI scans. Finally, we preliminarily demonstrated GlucoCEST MRI in five pregnant subjects who received a glucose tolerance test. For human studies, the dynamic R2* change was captured with T2*-weighted echo planar imaging (EPI). RESULTS: The GCE effect peaks at a saturation B1 field of about 2 μT, and the GlucoCEST effect increases linearly with the glucose concentration between 4-20 mM. In ex vivo tissue, the GlucoCEST MRI was sensitive to the glucose perfusate and the placenta vascular density. Although the in vivo GCE baseline was sensitive to field inhomogeneity and motion artifacts, the temporal evolution of the GlucoCEST effect showed a consistent and positive response after oral glucose tolerance drink. CONCLUSIONS: Despite the challenges of placental motion and field inhomogeneity, our study demonstrated the feasibility of DGE placenta MRI at 3.0 Tesla. 2019 Quantitative Imaging in Medicine and Surgery. All rights reserved.
BACKGROUND: To investigate dynamic glucose enhanced (DGE) chemical exchange saturation transfer (CEST) MRI as a means to non-invasively image glucose transport in the human placenta. METHODS: Continuous wave (CW) CEST MRI was performed at 3.0 Tesla. The glucose contrast enhancement (GCE) was calculated based on the magnetization transfer asymmetry (MTRasym), and the DGE was calculated with the positive side of Z-spectra in reference to the first time point. The glucose CEST (GlucoCEST) was optimized using a glucose solution phantom. Glucose solution perfused ex vivo placenta tissue was used to demonstrate GlucoCEST MRI effect. The vascular density of ex vivo placental tissue was evaluated with yellow dye after MRI scans. Finally, we preliminarily demonstrated GlucoCEST MRI in five pregnant subjects who received a glucose tolerance test. For human studies, the dynamic R2* change was captured with T2*-weighted echo planar imaging (EPI). RESULTS: The GCE effect peaks at a saturation B1 field of about 2 μT, and the GlucoCEST effect increases linearly with the glucose concentration between 4-20 mM. In ex vivo tissue, the GlucoCEST MRI was sensitive to the glucose perfusate and the placenta vascular density. Although the in vivo GCE baseline was sensitive to field inhomogeneity and motion artifacts, the temporal evolution of the GlucoCEST effect showed a consistent and positive response after oral glucose tolerance drink. CONCLUSIONS: Despite the challenges of placental motion and field inhomogeneity, our study demonstrated the feasibility of DGE placenta MRI at 3.0 Tesla. 2019 Quantitative Imaging in Medicine and Surgery. All rights reserved.
Entities:
Keywords:
Dynamic glucose enhancement; MRI; glucose chemical exchange saturation transfer (GlucoCEST); placental function
Authors: R J Moore; B K Strachan; D J Tyler; K R Duncan; P N Baker; B S Worthington; I R Johnson; P A Gowland Journal: Placenta Date: 2000-09 Impact factor: 3.481
Authors: Helene Benveniste; Joanna S Fowler; William D Rooney; Daryn H Moller; W Walter Backus; Donald A Warner; Pauline Carter; Payton King; Bruce Scharf; David A Alexoff; Yeming Ma; Paul Vaska; David Schlyer; Nora D Volkow Journal: J Nucl Med Date: 2003-09 Impact factor: 10.057
Authors: Rachel J Moore; Stephen S Ong; Damian J Tyler; Rachel Duckett; Philip N Baker; William R Dunn; Ian R Johnson; Penny A Gowland Journal: NMR Biomed Date: 2008-05 Impact factor: 4.044
Authors: Xiang Xu; Kannie W Y Chan; Linda Knutsson; Dmitri Artemov; Jiadi Xu; Guanshu Liu; Yoshinori Kato; Bachchu Lal; John Laterra; Michael T McMahon; Peter C M van Zijl Journal: Magn Reson Med Date: 2015-09-25 Impact factor: 4.668
Authors: Nirbhay N Yadav; Jiadi Xu; Amnon Bar-Shir; Qin Qin; Kannie W Y Chan; Ksenija Grgac; Wenbo Li; Michael T McMahon; Peter C M van Zijl Journal: Magn Reson Med Date: 2014-06-27 Impact factor: 4.668
Authors: Mina Kim; Afroditi Eleftheriou; Luca Ravotto; Bruno Weber; Michal Rivlin; Gil Navon; Martina Capozza; Annasofia Anemone; Dario Livio Longo; Silvio Aime; Moritz Zaiss; Kai Herz; Anagha Deshmane; Tobias Lindig; Benjamin Bender; Xavier Golay Journal: MAGMA Date: 2022-01-15 Impact factor: 2.310