Chrysanthemum (Chrysanthemum morifolium) CmICE2 conferred freezing tolerance in Arabidopsis.

Genes of the ICE (Inducer of CBF Expression) family play a key role in cold and freezing stresses response via the CBF regulatory pathway. In this work, we identified the ICE family gene, CmICE2, from Chrysanthemum morifolium 'Jinba'. CmICE2 encodes a 451-amino acid protein with a conserved nuclear localization domain, a bHLH domain and ACT domain. CmICE2 is expressed in abundance in leaves and flowers, and the expression of CmICE2 is induced by freezing and drought stresses. CmICE2 localized to the nucleus, and has transcriptional activity in yeast cells. After a 24-hour 4 °C acclimation, Arabidopsis plants overexpressing CmICE2 were more tolerant to freezing stress (-9 °C for 6 h) than the Col-0. When exposed to -9 °C for 6 h, the expression levels of genes such as AtCBF1, AtCBF2, AtCBF4, AtCOR 6.6A, AtCOR 414 and AtKIN1 were up-regulated significantly in CmICE2 overexpression plant lines compared to wild type. The proline contents, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were also increased in plants overexpressing CmICE2. In summary, CmICE2 confers to plant response to freezing stress.