Effect of an additional N-terminal methionyl residue on enzymatic and antifungal activities of Ageritin purified from Agrocybe aegerita fruiting bodies.

Ageritin, a specific ribonuclease, damaging the largest rRNA in the highly conserved α-sarcin/ricin stem-loop (SRL) has been well characterized from edible mushroom Agrocybe aegerita. Given its peculiar characteristic, Ageritin is the prototype of a new ribotoxins family expressed in basidiomycetes. In this framework, we report the characterization of Met-Ageritin, an isoform of Ageritin with an additional N-terminal methionyl residue. This difference affects the enzymatic features of this toxin despite is able to release α-fragment when acting on yeast, rabbit or Trichoderma asperellum ribosomes. Met-Ageritin inhibits protein synthesis in vitro with an IC50 = 2.8 nM that is 21-fold lower than that of Ageritin, while not show endonuclease activity on DNA. Subsequently, we explored the antifungal activity of both isoforms against T. asperellum, pathogen for A. aegerita and Saccharomyces cerevisiae, used as eukaryotic model microorganism. The presence of an additional N-terminal methionyl residue in Met-Ageritin abolishes antifungal activity towards T. asperellum, while neither of two isoforms is able to inhibit the growth of S. cerevisiae. Overall, these data highlight the importance of the N-terminal region of this toxin that likely alters the conformational state of this enzyme considering the presence in this region of metal binding sites necessary for explicate enzymatic activity.