Ahmet Savcı 1 , Enver Fehim Koçpınar 2 , Harun Budak 3 , Mehmet Çiftci 1 , Melda Şişecioğlu 3 Show Affiliations »
Abstract
BACKGROUND: Free radicals lead to destruction in various organs of the organism. The improper use of antibiotics increases the formation of free radicals and causes oxidative stress. OBJECTIVE: In this study, it was aimed to determine the effects of gentamicin, amoxicillin, and cefazolin antibiotics on the mouse heart. METHODS: 20 male mice were divided into 4 groups (1st control, 2nd amoxicillin, 3rd cefazolin, and 4th gentamicin groups). The mice in the experimental groups were administered antibiotics intraperitoneally at a dose of 100 mg / kg for 6 days. The control group received normal saline in the same way. The gene expression levels and enzyme activities of SOD, CAT, GPx, GR, GST, and G6PD antioxidant enzymes were investigated. RESULTS: GSH levels decreased in both the amoxicillin and cefazolin groups, while GR, CAT, and SOD enzyme activities increased. In the amoxicillin group, Gr, Gst, Cat, and Sod gene expression levels increased. CONCLUSION: As a result, it was concluded that amoxicillin and cefazolin caused oxidative stress in the heart, however, gentamicin did not cause any effects. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
BACKGROUND: Free radicals lead to destruction in various organs of the organism. The improper use of antibiotics increases the formation of free radicals and causes oxidative stress. OBJECTIVE: In this study, it was aimed to determine the effects of gentamicin , amoxicillin , and cefazolin antibiotics on the mouse heart. METHODS: 20 male mice were divided into 4 groups (1st control, 2nd amoxicillin , 3rd cefazolin , and 4th gentamicin groups). The mice in the experimental groups were administered antibiotics intraperitoneally at a dose of 100 mg / kg for 6 days. The control group received normal saline in the same way. The gene expression levels and enzyme activities of SOD, CAT , GPx , GR, GST, and G6PD antioxidant enzymes were investigated. RESULTS: GSH levels decreased in both the amoxicillin and cefazolin groups, while GR, CAT , and SOD enzyme activities increased. In the amoxicillin group, Gr, Gst, Cat , and Sod gene expression levels increased. CONCLUSION: As a result, it was concluded that amoxicillin and cefazolin caused oxidative stress in the heart, however, gentamicin did not cause any effects. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities: Chemical
Gene
Species
Keywords:
Antibiotic; GSH levels; antioxidant; enzyme activity; gene expression; oxidative stress
Mesh： See more »
Substances： See more »
Year: 2020
PMID： 31721686 DOI： 10.2174/0929866526666191112125949
Source DB: PubMed Journal: Protein Pept Lett ISSN： 0929-8665 Impact factor: 1.890