Doori Park1, Ik-Young Choi2, Nam-Soo Kim3. 1. Department of Agriculture and Life Industry, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, Republic of Korea. 2. Department of Molecular Bioscience, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, Republic of Korea. choii@kangwon.ac.kr. 3. Department of Agriculture and Life Industry, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, Republic of Korea. kimnamsu@kangwon.ac.kr.
Abstract
BACKGROUND: Various kinds of transposable elements (TEs) constitute high proportions of eukaryotic genomes. Although most of these TEs are not actively mobile, genome stress can induce mobilization of dormant TEs. Transgenic plants undergo tissue culture and subsequent whole-plant regeneration, which can cause genomic stress and in turn induce mobilization of inactive TEs. OBJECTIVES: To investigate the activation of transposable elements on the genome wide of the GM plant. METHODS: Transposon activities were analyzed in three transgenic rice plants carrying the insect resistance gene Cry1Ac and an herbicide resistance gene by the transposon display technique. These three transgenic plants were derived from a leading Korean rice variety, Illmi. RESULTS: We detected seven mobile activities in the mPing element, which is a MITE family transposon. The identity of the novel fragments in the gel display was confirmed by checking TAA target site duplication via sequence analysis. The genomic integration sites were all on different chromosomes, and the integrations were specific to either one or two T1 transgenic lines, except for one common integration on chromosome 4. One integration was in the 5'-UTR of the Glycerol-3-phosphate acyltransferase 8 gene, two integrations were in introns of expressed genes, and the other four integrations were in intergenic regions. CONCLUSION: Thus, novel mobilization of dormant TEs occurs in transgenic plants, which must be considered in the generation of genetically modified crops (GM crops).
BACKGROUND: Various kinds of transposable elements (TEs) constitute high proportions of eukaryotic genomes. Although most of these TEs are not actively mobile, genome stress can induce mobilization of dormant TEs. Transgenic plants undergo tissue culture and subsequent whole-plant regeneration, which can cause genomic stress and in turn induce mobilization of inactive TEs. OBJECTIVES: To investigate the activation of transposable elements on the genome wide of the GM plant. METHODS: Transposon activities were analyzed in three transgenic rice plants carrying the insect resistance gene Cry1Ac and an herbicide resistance gene by the transposon display technique. These three transgenic plants were derived from a leading Korean rice variety, Illmi. RESULTS: We detected seven mobile activities in the mPing element, which is a MITE family transposon. The identity of the novel fragments in the gel display was confirmed by checking TAA target site duplication via sequence analysis. The genomic integration sites were all on different chromosomes, and the integrations were specific to either one or two T1 transgenic lines, except for one common integration on chromosome 4. One integration was in the 5'-UTR of the Glycerol-3-phosphate acyltransferase 8 gene, two integrations were in introns of expressed genes, and the other four integrations were in intergenic regions. CONCLUSION: Thus, novel mobilization of dormant TEs occurs in transgenic plants, which must be considered in the generation of genetically modified crops (GM crops).
Authors: Ning Jiang; Zhirong Bao; Xiaoyu Zhang; Hirohiko Hirochika; Sean R Eddy; Susan R McCouch; Susan R Wessler Journal: Nature Date: 2003-01-09 Impact factor: 49.962
Authors: Yoshihiro Kawahara; Melissa de la Bastide; John P Hamilton; Hiroyuki Kanamori; W Richard McCombie; Shu Ouyang; David C Schwartz; Tsuyoshi Tanaka; Jianzhong Wu; Shiguo Zhou; Kevin L Childs; Rebecca M Davidson; Haining Lin; Lina Quesada-Ocampo; Brieanne Vaillancourt; Hiroaki Sakai; Sung Shin Lee; Jungsok Kim; Hisataka Numa; Takeshi Itoh; C Robin Buell; Takashi Matsumoto Journal: Rice (N Y) Date: 2013-02-06 Impact factor: 4.783