Ahmet Özbilgin1, Gulsum Gencoglan2, Varol Tunali3, İbrahim Çavuş1, Ahmet Yıldırım1, Cumhur Gündüz4, Mehmet Harman5. 1. Department of Parasitology, Faculty of Medicine, ManisaCelal Bayar University, Manisa, Turkey. 2. Department of Dermatology, Faculty of Medicine, ManisaCelal Bayar University, Manisa, Turkey. 3. Department of Parasitology, Faculty of Medicine, Ege University, Inisdibi Cad. Nirvana YasamEvleri 25/C Daire:6Akyaka Ula Mugla, Izmir, Turkey. varoltunali@gmail.com. 4. Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey. 5. Department of Dermatology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Abstract
PURPOSE: Due to mass population movements driven by internal conflicts and wars, cutaneous leishmaniasis (CL) is becoming increasingly important in Turkey. This study is aimed at determining the clinical aspects, diagnosis and genotyping of CL patients coming to Turkey from abroad. METHODS: In our study, the clinical materials obtained from the patients or sent for diagnostic purposes from other centers to our laboratory between years 2012 and 2016 were assessed retrospectively. In total, there were 38 patients from Syria, Iraq, Afghanistan, Iran, and Turkmenistan. RESULTS: 29 (76%), 28 (73%) and 33 (87%) samples were positive by light microscopy, Novy-McNeal-Nicolle(NNN), and enriched medium, respectively. By ITS-1 gene region PCR, 31 (81%) of the cases were positive. 35 of the patients were tested positive by at least one of the diagnostic methods. By genotyping, 21 Leishmania tropica, 8 Leishmania major, 3 Leismania infantum, 2 Leishmania donovani, and 1 Leishmania aethopica were detected. CONCLUSION: This study is aimed at informing the clinicians working in the field for the import CL cases and recording the changing epidemiological features of CL in the region as well as discussing the possible focus for L. aethiopica infection which has not been shown in the region before.
PURPOSE: Due to mass population movements driven by internal conflicts and wars, cutaneous leishmaniasis (CL) is becoming increasingly important in Turkey. This study is aimed at determining the clinical aspects, diagnosis and genotyping of CLpatients coming to Turkey from abroad. METHODS: In our study, the clinical materials obtained from the patients or sent for diagnostic purposes from other centers to our laboratory between years 2012 and 2016 were assessed retrospectively. In total, there were 38 patients from Syria, Iraq, Afghanistan, Iran, and Turkmenistan. RESULTS: 29 (76%), 28 (73%) and 33 (87%) samples were positive by light microscopy, Novy-McNeal-Nicolle(NNN), and enriched medium, respectively. By ITS-1 gene region PCR, 31 (81%) of the cases were positive. 35 of the patients were tested positive by at least one of the diagnostic methods. By genotyping, 21 Leishmania tropica, 8 Leishmania major, 3 Leismania infantum, 2 Leishmania donovani, and 1 Leishmania aethopica were detected. CONCLUSION: This study is aimed at informing the clinicians working in the field for the import CL cases and recording the changing epidemiological features of CL in the region as well as discussing the possible focus for L. aethiopicainfection which has not been shown in the region before.
Authors: Ahmet Özbilgin; Mehmet Harman; Mehmet Karakuş; Aldert Bart; Seray Töz; Özgür Kurt; İbrahim Çavuş; Erdal Polat; Cumhur Gündüz; Tom Van Gool; Yusuf Özbel Journal: Acta Trop Date: 2017-06-03 Impact factor: 3.112
Authors: E Chouihi; F Amri; N Bouslimi; E Siala; K Selmi; N Zallagua; R Ben Abdallah; A Bouratbine; K Aoun Journal: Pathol Biol (Paris) Date: 2008-05-05